Abstract:Objective To study the effect of intratubular injection of busulfan on ablation of pig endogenous spermatogonial stem cells (SSCs) and allotransplantation of SSCs on the recovery of the reproductive ability of the recipient after ablation of endogenous SSCs.Method Nine 6-week-old Large-white boars were injected through seminiferous tubules with busulfan at a dosage of 3 mg/kg, and other three pigs were injected with 2 mL DMSO as controls. After three weeks, each boar in the test group was given a second injection at the same dosage. At three weeks after the second injection, testes from test and control groups were collected to evaluate the endogenous SSC ablation conditions. At one month after the second busulfan injection, testicular single-cell suspension was isolated from the testes of 5?7-day-old Large-white piglets by two-step enzyme digestion method and purified by gelatin differential adherence method. After purification, the purity of SSCs was analyzed by immunofluorescence and flow cytometry. At four months after allotransplantation of SSCs, microsatellite marker analysis was used to detect the presence of donor-derived SSCs in recipient boar semen and testicular tissue.Result After treating the Large-white boar twice with busulfan at a dosage of 3 mg/kg, the results of HE staining and immunohistochemistry staining of testis tissue showed that germ cells at all levels in the seminiferous tubules of the testes in test group were ablated but the structure of sertoli cell was intact, which could support the colonization and development of transplanted exogenous SSC. The results of immunofluorescence and flow cytometry showed that the rate of UCHL-1 positive cells in the isolated testicular single-cell suspension increased from 16.3% before differential adhesion to 50.8% after purification. At four months after allotransplantation of porcine SSC, HE staining and immunohistochemical staining of testicular tissues in the transplanted group showed recovery of germ cell layers compared with the group injected with busulfan but not transplanted with donor cells. UCHL-1 positive SSCs were detected on the basement membrane of seminiferous tubules in recipient testes. Microsatellite marker analysis of recipient testis tissue showed the presence of donor SSCs, suggesting that donor SSCs transplanted into recipient testes could colonize and survive in recipient testes for more than four months. However, microsatellite marker analysis of semen did not detect donor-derived sperms.Conclusion Intratubular injection of busulfan into boar testes at a dosage of 3 mg/kg can effectively ablate endogenous SSCs and can be used to prepare recipient pigs for SSC transplantation. Two-step enzyme digestion and gelatin differential adhesion method can be used to successfully isolate and purify porcine SSCs. Porcine SSCs can colonize in recipient testes and survive for more than four months after allotransplantation.

Abstract:Objective To investigate the genetic variation of astrovirus identified in the kidney of a dead Alopex lagopus in Guangzhou Zoo.Method The autopsy was performed on the dead A. lagopus, and the etiology detection of the internal organs was determined by semi-nested RT-PCR. The amplified RdRp gene sequence of the virus strain was used for similarity analysis.Result The kidney of the dead A. lagopus were pale, enlarged and the capsule was difficult to be stripped off. The astrovirus RdRp gene in kidney showed positive. The astrovirus RdRp gene had nucleotide similarities of 67.5%?96.2% with nine referenced astrovirus strains from other animals, and was most closely related to the feline astrovirus 2 strain 1637F isolated in Hong Kong (96.2% similarity).Conclusion The study enlarges the knowledge of the interspecies transmission of astrovirus among wild mammals, also proves the possibility of extraintestinal infection caused by astrovirus.

Abstract:Objective To evaluate the effect of orally administrated self-developed chlortetracycline microcapsule on microflora quantity in sheep rumen.Method Eighteen sheep of (29.0±2.3) kg of 6-month-old with good health condition were randomly assigned to six groups with three replicates in eash grcup, including one control group and five medicated groups. The sheep in the control group were only fed with basal diet. The treatments in five medicated groups were as follows. Group I: Basal diet supplemented with normal chlortetracycline premix granule; Group II: Basal diet supplemented with hydrogenated oil prescription chlortetracycline microcapsule; Group III: Basal diet supplemented with PEG4000/glycerol stearate (mass ratio of 5︰5) prescription chlortetracycline microcapsule; Group IV: Basal diet supplemented with hydrogenated oil/glycerol stearate (mass ratio of 9︰1) prescription chlortetracycline microcapsule; Group V: Basal diet supplemented with hydrogenated oil/glycerol stearate (mass ratio of 8︰2) prescription chlortetracycline microcapsule. The chlortetracycline administration dosages of sheep in the five medicated groups were all 25 mg/kg. The sheep were administrated once a day before morning feeding for five days. The rumen contents were collected by rumen fluid collector and quickly filtered through four layers of gauze after continuous administration. The filtrate were collected in the cryopreserved tube and quickly frozened by liquid nitrogen then stored at ?80 ℃. The rumen microorganisms of liquid phase were quantitatively detected by real-time fluorescence quantitative PCR (RT-PCR). Result Compared with the normal chlortetracycline premix granule, hydrogenated oil/glycerol stearate (mass ratio of 9︰1) prescription chlortetracycline microcapsule had better effect, only significantly increased the quantity of Fibrobacter succinogens, and had no significant influence on the quantities of other microbial flora in sheep rumen. Compared with the control group, hydrogenated oil/glycerol stearate (mass ratio of 9︰1) prescription chlortetracycline microcapsule and PEG4000/glycerol stearate (mass ratio of 5︰5) prescription chlortetracycline microcapsule had relatively better effects, significantly decreased the quantities of Streptococcus bovis and F. succinogens respectively, and had no significant influence on the quantities of other microbial flora in sheep rumen.Conclusion The hydrogenated oil/glycerol stearate (mass ratio of 9︰1) prescription chlortetracycline microcapsule has less inhibition effect on rumen microflora quantity and meets clinical application requirements.

Abstract:Objective To study the effect of histidine kinase gene envZ in two-component system ompR/envZ on biofilm formation ability of avian pathogenic Escherichia coli (APEC), understand the regulation mechanism of the two-component system on biofilm of APEC, and provide a reference for investigating the ways of biofilms affecting APEC antibiotic resistance.Method The red homologous recombination method was used to construct the envZ-gene-deleted strain, and the differences in growth characteristics and biofilm formation abilities between wild strain and the envZ-gene-deleted strain were compared. The mechanism of envZ regulating biofilm formation related genes in transcriptional regulatory network was analyzed by transcriptomics.Result The envZ-gene-deleted strain AE17ΔenvZ was successfully constructed. The envZ gene deletion had no significant effect on growth rate of APEC, but weakened the APEC biofilm formation ability. Compared with wild strain AE17, AE17ΔenvZ had 711 differentially expressed genes, and the genes related to biofilm formation were significant down regulation.Conclusion In addition to responding to environmental osmotic pressure, envZ gene is also involved in regulating the formation of APEC biofilm.

Abstract:Objective To establish a method of high performance liquid chromatography (HPLC) with a photodiode array detector (PDA) for the determination of ponazuril in pig feces and urine.Method Urine samples were extracted twice with 0.2%(φ) acetic acetonitrile and dichloromethane. Feces samples were vortex-extracted by acetonitrile and purified by hydrophile-lipophile balance (HLB) solid phase extraction column. The mobile phase was 0.005 mol/L potassium dihydrogen phosphate solution (A)-acetonitrile (B), the mobile phase ratios of V(A)∶V(B) for urine and feces samples were 55∶45 and 56∶44 respectively. The detection wavelength was 255 nm, the column temperature was 35 ℃ and the injection volume was 30 μL.Result The detection limit and quantitative limit of ponazuril in urine were 0.02 and 0.05 μg/mL, respectively, which showed a good linear relationship in the range of 0.05?5.00 μg/mL, and the determination coefficients (R²) was 0.999 8. The average recovery rates ranged from 93.49% to 99.16% at three spiked levels of 0.05, 1.00 and 5.00 μg/mL, and the intra-batch and inter-batch relative standard deviations (RSDs) ranged from 0.97% to 7.62%. The detection limit and quantitative limit of ponazuril in feces were 0.10 and 0.25 μg/g, respectively, which showed a good linear relationship within the range of 0.25?100.00 μg/g, and R² was 0.999 5. The average recovery rates ranged from 89.55% to 95.88% at three spiked levels of 0.25, 25.00 and 100.00 μg/g, and the intra-batch and inter-batch RSDs ranged from 1.76% to 3.63%. The recovery rates of ponazuril in feces and urine were both higher than 89.50%, and the intra-batch and inter-batch RSDs were both lower than 8%.Conclusion This method has simple sample pretreatment and sensitive detection, and is suitable for the determination of ponazuril in pig excrement.

Abstract:Objective To develop a new formulation of enrofloxacin nanoemulsion for spray administration, and evaluate its disease control effect using chicken model of systemic infection.Method The phase transition method was used, and single factor and orthogonal tests were performed to screen formulations and optimize the prescription. High-performance liquid chromatography (HPLC) was used to determine enrofloxacin content in nanoemulsion. Nanoparticle and zeta potential analyzer was used to measure the particle size and zeta potential of nanoemulsion. Pharmacodynamic experiments were performed using chicken models infected by Salmonella pullorum and Escherichia coli, and the control effects of spray adminstration of compound enrofloxacin nanoemulsion on bacterial diseases were evaluated.Result The nanoemulsion prepared in this experiment was an oil-in-water type, light yellow, clear and transparent, and had uniform droplet distribution. The average particle size was (10.79 ± 1.5) nm, the polydispersity index (PDI) was 0.446 and the zeta potential was −21.63 mV. The pharmacodynamic evaluation showed that the survival rates of preventive administration groups with high, medium and low doses were 90.0%, 83.3% and 73.3% respectively, which were significantly higher than that of the infection control group (26.7%) (P<0.01).Conclusion The compound enrofloxacin nanoemulsion prepared in this study has stable properties. The ultrasonic spray administration has good control effects on chicken diseases due to systemic infection of Salmonella pullorum and Escherichia coli.

Abstract:Objective To compare the biological effects and variation frequency of two mutagenesis methods, including space mutation and heavy ion radiation, in different generations of rice (Oryza sativa L.), and provide some methods and theoretical guidances for rice mutation breeding.Method The dry seeds of pure line rice variety ‘Huahang 31’ were treated using space mutation and different dose of heavy ion radiation, and the seeds without mutation treatment were used as the control. The phenotypic and cytological mutagenic effects of the 1st generation of mutation (M₁) were analyzed. The amylose content and grain shape trait of the 2nd generation of mutation (M₂) were screened by phenotypic and genotypic targeted screening. The variation frequencies in M₂ of two mutation treatments were compared.Result The seed vigor index of space mutation M₁ was 14.62% lower than that of the control. The seed vigor index of heavy ion radiation M₁ showed a saddle effect curve with the increase of radiation dose, and the seed vigor index of 10 Gy heavy ion radiation M₁ was 14.92% lower than that of the control which was like the mutation effect of space mutation. The grain type and amylose content mutation frequencies of space-induced M₂ were 4.14% and 1.61% respectively, while the grain type and amylose content mutation frequencies of 80 Gy heavy ion radiation were 4.88% and 1.55% respectively. When HRM technology was used to scan the 673 bp sequences of four intervals of Wx gene, three SNP mutations were found in 4 736 samples of space-induced with a mutation density of 1/1063.83 kb. Four SNP mutations were found in 4 848 samples of heavy ion radiation with a mutation density of 1/815.68 kb.Conclusion Two kinds of mutagenic treatments can induce the character variations of rice. The physiological effects of space-induced mutagenesis M₁ is similar to low-dose heavy ion radiation, and the mutation frequency of M₂ is similar to high-dose heavy ion radiation.

Abstract:Objective To investigate the influence of fruit maturity at harvest, tree vigour and preharvest regulation on the storage performance and shelf life of Dimocarpus longan fruit.Method D. longan Lour. cv. Shixia fruits were used as experimental material. Three groups of single factor experiments were designed with fruit maturity, tree vigour and preharvest regulation as the experimental factors, and each factor had three levels. The harvested fruits were treated using SO₂-released paper and were stored at (5±1) ℃ for 50 days. We observed the good fruit rate after storage (STGFR), and the good fruit rate during shelf life (SFGFR) at (25±1) ℃. The storage performances of fruits with different preharvest treatments were compared.Result Fruits harvested at appropriate maturity (80%?85% maturity degree) had higher STGFR and SFGFR than those at full maturity (95%?100% maturity degree, CK) and over maturity, and the over matured fruits performed the worst. Both preharvest disease control and nutrition regulation treatments resulted in fruits with higher STGFR and SFGFR compared to CK with significant differences (P<0.01). Among them, disease control treatment performed the best with 93.26% STGFR, followed by nutrition regulation treatment (84.23%), and the worst was CK (67.71%). Among three types of trees with different vigor, fruits from strong tree had the best storability, followed by weak tree, and CK(medium tree) was the worst. Among all treatments, the fruit storability of disease control treatment was the best, with a SFGFR of 84.86% after four days on shelf, followed by strong tree, nutrition regulation and week tree treatments, with SFGFR of 63.23%, 56.14% and 51.12% respectively, and the worst was the over matured fruit (26.91%). The results of correlation analysis indicated that the content of total soluble solids in fruit pulp at harvest was significantly negatively correlated with the amount of SO₂ residue in pulp and mass loss rate during shelf life, but not correlated with storability.Conclusion During the fruit growth period of D. longan, performing disease control and nutrition regulation, keeping reasonable tree vigour with suitable fruit setting rate, and harvesting at appropriate maturity can help the fruit obtain better storability and longer shelf life.

Abstract:Objective To study the spatial distribution of soil organic matter in Mayi lake, the water source of the eastern ecological barrier of Karamay city, and provide scientific basis and data support for the ecological environment protection of the lake area.Method The study area of this paper was Mayi lake. Through field sampling and indoor analysis, the study used trend analysis method, inverse distance weight interpolation method, spatial autocorrelation method and semi-variogram function method to analyze the spatial distribution law of soil organic matter at different depths of Mayi lake.Result The trend analysis showed that the change rates of soil organic matter content in Mayi lake were different from 0?20, 20?40, 40?60 and 60?80 cm soil layers, but the overall trend was that soil organic matter content increased in the north-south direction and decreased in the east-west direction. The inverse distance weight interpolation method (IDW) showed that there are large horizontal distribution differences of soil organic matter contents in different soil layers, and regional obvious vertical distribution characteristics of soil organic matter content in Mayi lake. The trends in soil organic matter content change were highly consistent with trend analysis method. The overall performance of the north-south direction of soil organic matter content increased, the east-west direction showed a trend of decrease. The spatial autocorrelation method showed that Moran indexes of four layers were 0.164 3, 0.123 6, 0.195 5 and 0.246 1 respectively, showing a significant positive correlation on the space. The Z values of 0?20, 20?40, 40?60 and 60?80 cm soil layers were 3.151 0, 2.593 4, 3.590 3 and 4.635 5 respectively. The soil organic matter of underlayers (40?60, 60?80 cm) had obvious positive spatial correlation with the highest space aggregation degree. The surface layers (0?20, 20?40 cm) had no significant spatial correlation, and low spatial aggregation degree. The semi-variogram function method showed that the nugget effects of the underlayers (40?60 and 60?80 cm) were 0.427 and 0.420 respectively, indicating spatial correlation of soil organic matter was general. The nugget effects of the surface layers (0?20 and 20?40 cm) were 0.033 and 0.045 respectively, indicating high spatial correlation of soil organic matter. Conclusion The contents of soil organic matter in different soil layers vary greatly, and the north-south direction tends to increase, while the east-west direction tends to decrease. The spatial correlations of soil organic matter in the surface layers (0−20 and 20−40 cm) are not significant, and the spatial aggregation degrees are low, while the spatial correlations are significant and the spatial aggregation degrees are high in the underlayers (40−60 and 60−80 cm). The spatial heterogeneity of soil organic matter content is greatly affected by soil type, soil texture, vegetation type and the variation of lake area. While constructing ecological barrier in the periphery of lake area, the selection of shelterbelt tree species, planting depth and planting density should be combined with the spatial distribution of soil organic matter content.

Abstract:Objective To determine the resistance of rice variety ‘Guiyu 11’ to brown planthopper, Nilaparvata lugens, and provide technical supports for green control of N. lugens.Method The resistance of ‘Guiyu 11’ to N. lugens was systematically evaluated using seedling screening test, honeydew excretion measurement, laboratory evaluation of adult-plant and field investigation of N. lugens populations.Result ‘Guiyu 11’ and the resistant variety ‘Rathu Heenati’ (as the control) showed moderate resistance to N. lugens at seedling stage. The average quantity of honeydew secreted by N. lugens after feeding on ‘Guiyu 11’ for 24 hours was not significantly different from that of ‘Rathu Heenati’ at tillering and booting stages. At adult-plant stage, ‘Guiyu 11’ and ‘Rathu Heenati’ plants grew normally after infected by N. lugens for 15 days, while all plants of the sensitive variety ‘TN1’ withered and died. The field investigation of dynamic insect density showed that the average numbers of N. lugens on ‘Guiyu 11’ on June 19 and June 24 in 2019 were 218.33 and 436.67 per hundred clump, respectively, and were significantly lower than that on the sensitive variety ‘Liushayouzhan 202’.Conclusion ‘Guiyu 11’ is moderately resistant to N. lugens and shows significant inhibiting effect on the growth of N. lugens population in paddy field. ‘Guiyu 11’ can be applied to the ecological control of N. lugens.

Abstract:Objective To determine the control effect of sorghum vinasse against broomrape (Orbanche cumana) and the influence of vinasse on growth and quality of tobacco.Method A field plot assay was conducted and different dosages of vinases were applied in soil. The control effect of vinasse against broomrape and influence on growth, yield and quality of tobacco were investigated.Result Vinasse had good control effects against broomrape at different dosages. The average plant control effect and average fresh weight control effect of vinasse against broom rape were 62.73%−74.16% and 42.70%−66.03% respectively in 2018, and 57.10%−64.17% and 53.23%−80.56% respectively in 2019. Tobacco grew well with the application of different dosages of vinasse, and the growth indicators were significantly higher than those of the control. Tobacco yield increased significantly after applying vinasse, fresh weights increased by 17.76%−24.22% and 6.63%−15.71% than those of the control, and dry weight increased by 6.70%−11.63% and 15.39%−20.17% in 2018 and 2019, respectively. The proportion of superior and middle class of tobacco increased by 7.35% and 3.90% than those of control, in 2018 and 2019 respectively.Conclusion Sorghum vinasse can be used for the prevention and control of tobacco broomrape. It is suggested that vinasse mixed with soil in a depth of 20 cm at the dose of 1−2 kg/m² before tobacco seedling transplanting.

Abstract:Objective To identify autophagy-related genes (Atg) in fall armyworm (Spodoptera frugiperda) genome and their response to viral infection in S. frugiperda, and provide a basis for further research on the molecular mechanism of autophagy.Method Autophagy-related protein (ATG) sequences from various species, such as human (Homo sapiens) and fruit fly (Drosophila melanogaster), were aligned and analyzed for sequence similarity. ATG sequences in S. frugiperda genome were searched. Conservative domain (CD) of the candidate ATG were analyzed by NCBI CD search. Furthermore, qRT-PCR was used to detect the expression changes of Atgs in Sf9 cells after infection by recombinant EGFP-AcMNPV.Result We identified more than ten core Atgs which were involved in autophagosome formation in S. frugiperda, including the complete sequences of Atg3, Atg5, Atg6, Atg7, Atg10, Atg12, Atg13 and Atg101, and the partial sequences of Atg1, Atg2, Atg4, Atg9, Atg14, Atg16, Atg17 and Atg18. The expression levels of S. frugiperda Atg2, Atg4, Atg5, Atg6, Atg7, Atg8 and Atg12 were up-regulated after 6 to 12 hours infection by EGFP-AcMNPV.Conclusion Eukaryotic conserved Atgs exist in the genome of S. frugiperda, indicating the autophagy pathway in S. frugiperda is evolutionarily conserved. AcMNPV infection might trigger the autophagic response in Sf9 cells.

Abstract:Objective To investigate the relationships of Carterocephalus alcina Evans, 1939 and its relatives.Method The relationships of C. alcina and its relatives were analyzed based on morphological characters, COI barcodes and geographical distribution using an extensive sample collection.Result C. alcinoides Lee, 1962 is only an individual variation of C. alcina, and therefore it is a new synonym of C. alcina. The minimum mean genetic distance between the two samples from Beijing and relatives is 3.9%, and they form a distinct clade in the phylogenetic tree. They are characterized by the presence of spots at the base of the discoidal cell of the forewing upperside and in the middle of the leading edge of the hindwing as well as the spot of M_1-2 cells extending to the submarginal zone on the hindwing upperside. C. alcina, the clade composed of two samples from Beijing, C. palaemon and C. silvicola are recovered as a strongly supported monophyletic clade.Conclusion C. alcinoides is a new junior synonym of C. alcina. The population which has been known as C. alcina from Beijing is actually a new species, C. longimaculatus Hou, Fan & Li sp. nov..

Abstract:Objective To study the genetic variation of Eucalyptus urophylla, select excellent clones suitable for growing in Yangjiang, and provide a scientific basis for selection and promotion of new clones.Method Totally 76 E. urophylla clones were used as materials, and the clonal test fields of E. urophylla were constructed in three times. After afforestation, tree height and diameter at breast height (DBH) were measured every year, and the phenotypic traits were analyzed by variance analysis and genetic parameter estimation. The multi-trait genotype value was used to screen excellent clones, and the genetic gain of excellent clones was also estimated.Result Tree height and DBH of E. urophylla had significant differences among clones, and the variation coefficient of growth traits decreased year by year. Except for the traits of a few years, the variance component of E. urophylla growth traits reached a significant or extremely significant level among blocks, with genetic variance components ranging from 0.10 to 22.61, environmental variance components ranging from 0.18 to 45.86, and repeatability ranging from 0.10 to 0.42, respectively. There was strong positive genetic correlation between tree height and DBH except for tree height of one-year-old tree. Both genetic and phenotypic correlations between tree height and DBH reached medium to high levels with the correlation coefficients ranging from 0.40 to 0.98. The correlations between tree height and DBH at the same tree age were large. Based on the predicted tree height and DBH genotype values at different ages with the growth performance, excellent clones of ZQUB29, ZQUA34, ZQUC33, ZQUA15 and ZQUA33 had higher growth genotype and phenotype values at all ages, indicating that these clones may be more stable throughout the rotation period and were excellent clones.Conclusion The growth differences among E. urophylla clones involved in the fields are extremely significant, and five excellent clones can be used as the basic materials for the subsequent genetic improvement of E. urophylla and the breeding of improved varieties.

Abstract:Objective To comprehensively analyze the phenotypic variations in wild populations of Paris polyphylla var. chinensis (Franch.) Hara, and provide a theoretical support for the protection of germplasm resources, development and utilization of medicinal materials, and breeding improvement.Method The seven wild populations of P. polyphylla var. chinensis in China were used as materials. Based on the measurement of 17 phenotypic traits, by the analyses of variance, variation coefficient, multiple comparisons and correlation, the phenotypic variation and its relationship with geographical climate factors were discussed.Result Except the numbers of leaf and calyx, the other 15 phenotypic traits of wild P. polyphylla var. chinensis showed significant or extremely significant differences among and within populations. The variation within populations (Percentage of variance component was 52.19%) was greater than that among populations (Percentage of variance component was 26.39%). The mean phenotypic differentiation coefficient of phenotypic traits was 34.27% and the main source of variation was within populations. The average variation coefficient of each phenotypic trait was 17.72% and variation coefficient ranged from 6.83% to 29.95%. The variation ranges of peduncle length, leaf area, calyx area and plant height were higher than the average variation coefficients of these five traits, while those of leaf number, calyx number, fruit transverse diameter, fruit longitudinal diameter, fruit transverse diameter to longitudinal diameter ratio and seed 1000-grain weight were far less than the mean variation coefficients, indicating that the stabilities of flower and leaf quantitative trait indexes, fruit and seed traits were greater than those of leaf shape traits. Correlation analysis showed that most of the 17 phenotypic traits had significant or extremely significant correlations with the geographical and climatic factors.Conclusion The wild populations of P. polyphylla var. chinensis in Qingyuan City, Guangdong Province have the most abundant geographical population variations and can be used as the optimal target for germplasm collection and preservation.

Abstract:Objective To develop a kind of instant porridge with a lower glycemic index by the twin-screw extrusion processing using millet flour as the main raw material to mix with defatted soybean flour, and optimize the processing technology.Method The mix ratio of defatted soybean flour was determined according to the FAO/WHO standard model. Based on the single-factor test, the Box-Behnken Design (BBD) method was used to design and optimize the experiment. The effects of extrusion temperature, screw speed, material moisture content, and oven temperature on the quality of instant porridge were studied to finally determine the optimal processing technology condition.Result When the addition amount of defatted soybean flour was 30% (w), the essential amino acid scores were all greater than one after complementing with millet flour, which complied with the FAO/WHO standard mode. The optimal processing conditions were the extrusion temperature of 145 ℃, the screw speed of 220 r/min and the material moisture content of 35%. Under the optimized conditions, the product with higher gelatinization (86.72%), lower glycemic index (63.21), high rehydration rate (305%) and high sensory score (93 points) was obtained.Conclusion The process conditions have significant effects on the physicochemical property and eating quality of instant porridge, and their change law provides a theoretical basis for the development of other related products.