The growth and differentiation of the Callus of the Clove Gilly-flower were promoted when treated with laser beam.He-Ne laser(20 mw)made the callus grow rapidly and the fresh weight of callus tissue increased by 170% as compared with tfae control ,and the differentiation of bud was promoted remarkably after irradiation by Ar+laser (0.1w and 1w).Moreover,some special proteins emerged in calluses which were treated by laser beam.It seems that tfae promotion of the growth and differentiation of callus tissue are related to the appearance of certain proteins.