籼稻原生质体游离培养及再生植株
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THE ISOLATION, CULTURE AND PLANT REGENERATION OF 1NDICA RICE
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    摘要:

    籼稻(Oryza sativa L.)推广品种“秋桂矮11”成熟种胚在N6+2,4-D培养基上诱导选择胚性愈伤组织,然后转入N6+2,4-D及高浓度脯氨酸的液体培养基悬浮培养。继代近6个月后建成胚性细胞悬浮系.悬浮细胞通过酶解游离产生大量原生质体,经合适渗透压的KpR琼脂糖培养基包埋,在无哺育培养条件下,分裂频率为11.36%,细胞克隆转至N6分化培养基再生出完整绿色小植株,分化频率为9.4%。

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    Calli were induced from germinating seeds of indica rice ( Orgza saliva L. indica variety )" Qiuguiai 11" and the embryogenetic calli were selected and transferred to N6 liquid media in which embryogenetic cell suspension cultures were established after 6 months' subculture. Protoplasts were isolated from suspension cultures and imbedded in KpR agarose media. Without using nurse culture, the division frequency was 11.36%. The regenerated cell colonies were transferred onto N6 differentiation medium in which green plant formed. The plant regeneration frequency was 9. 4%.

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张伟 简玉瑜.籼稻原生质体游离培养及再生植株[J].华南农业大学学报,1993,(3):70-74

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