昆虫病毒包涵体电镜样品的制备及其超微结构观察
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THE PREPARATION FOR INCLUSION BODY VIRUS OF INSECT AND THE OBSERVATION OF ITS ULTRASTRUCTURE WITH ELECTRON MICROSCOPE
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    摘要:

    在提纯昆虫病毒包涵体及其病毒粒子的电镜样品制备进行了初步探讨.结果表明:在电镜下观察,包涵体及其病毒粒子图像清晰,显示了病毒粒子的形态特征,可达到昆虫病毒电镜鉴定之目的.与此同时,还观察到某些包涵体表面呈蜂窝状或沟型凹陷等“前多角体”的特殊构造;多角体块状蛋白质晶格、多角体外膜以及杆状病毒粒子蛋白亚基斜纹花样、病毒粒子囊膜等超微结构.

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    The purification of inclusion body virus of insect and the preparation for the virus sample for electron microscope were tentatively studied in this paper. The pictures of inclusion body and its virion under electron microscope were shown clearly,and displayed themorphological character of virion and made it possible to identify insect virus with electron microscope. The special structure such as cellular and ditch-like concave propolyhedra, ultrastructure such as polyhedron massive proteinic Crystal,Polyhedron membrane,and herringbone Pattern of Protein subunit of baculiform virion and envelope of virion were also observed clearly.

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石木标 戴冠群.昆虫病毒包涵体电镜样品的制备及其超微结构观察[J].华南农业大学学报,1996,(3):92-95

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