Floristic characteristics of the seed plants of Nanling National Nature Reserve were studied. A total of 2 292 species and varieties of the seed plants belonging to 822 genera, 175 families, was identified in Nanling National Nature Reserve. Among them, there are 30 species belonging to 20 genera, 9 families are of gymnosperm and 2 262 species and varieties belonging to 802 genera, 166 families are of angiosperm. The distribution elements of Nanling's flora are complex, diverse and widely related to many parts of the world. They comprise 14 of 15 distribution types of Chinese seed plants.The flora is predominated by tropical and subtropical elements and also strongly affected by temperate elements. There are 33 endemic genera. Nanling's flora has a long biological history. On the one hand, there are many primitive and relic plants, Nanling might be one of their original centers;on the other hand there are also many advanced plants, Nanling might be one of their specialization and distribution centers.