When the PVP(Polyvinylpyrrolidone) was used in pretreatment of sugareane explant and usedas an additive in medium, a positive result was obtained which prevented brown turning and necrosis ofinoculum. Meanwhile, it was found that the proliferous rate of differentiated bud was delayed when thePVP concentration in the medium increased in a certain extent. The application of PVP in the plant tissueculture was evaluated affirmatively.