甘蔗不同基因型组织培养特性的研究
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Studies on the Properties of Tissue Culture of Different Sugarcane Genotypes
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    摘要:

    通过甘蔗心叶的组织培养,研究了11个甘蔗基因型的组织培养特性。结果表明,用200mg/L的PVP溶液浸泡外植体(心叶)20min,明显降低了诱导培养过程中中外植体的褐化率,同时大大提高了各个基因型的愈伤组织诱导率,11个甘基因型在同样的培养条件下,外植体的褐化率,愈伤组织的诱导率,愈伤组织的增殖率,愈伤组织的分化率都表现出显著的差异。

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    Properties of tissue culture of 11 sugarcane genotypes were studied. The results showed that the ratios of explant browning were obviously decreased and the ratios of callus inducing were greatly increased after the explants were soaked in 200 mg/L polyvinylpyrrolidone(PVP) solution for 20 minutes. The ratios of explant browning, callus inducing, callus multiplication and callus differentiation of 11 sugarcane genotypes were significantly different in the same culture condition.

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梁计南,谭中文,谭志勇,张志胜,孔垂华,胡飞.甘蔗不同基因型组织培养特性的研究[J].华南农业大学学报,2002,23(4):37-40

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  • 最后修改日期:2001-08-07
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