用RNA干涉对几个水稻WD结构域基因的功能研究
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国家重点基础研究发展计划(973计划)


Functional Analysis of Several Rice WD Domain Genes by RNA Interference
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    为研究水稻中WD结构域基因的功能,构建了8个WD结构域基因的RNA干涉转化载体并转化水稻品种.其中6个基因获得了转化体,3个基因(代号为E10,G04,和G05)的转化体出现表型变异.E10和G04的转化植株表现为花柱数目增加,E10转化植株的花柱基部还长出1条半透明物体;G05的转化植株表现为花粉不育.对转化植株的靶基因进行RT-PCR检测表明,大部分转化植株的内源基因表达量降低或没有表达.这些结果表明,这些WD结构域基因的功能与生殖发育有关.

    Abstract:

    To study the functions of WD domain genes in rice,eight WD domain genes were selected from rice and used to prepare RNA interference constructs for transformation.Transgenic plants were obtained from six of the RNAi constructs and phenotypic variations were observed in the transgenic plants with three genes(coded as E10,G04,and G05).The transgenic plants of E10 and G04 exhibited increase of the number of stigmas,and a transparent unknown organ was arisen from the basis of the stigmas in the E10 transgenic plants.The transgenic plants of G05 showed male sterility.The expressions of the targeted genes in the transgenic plants were suppressed as assayed by RT-PCR.These results show that the WD domain gene's functions in rice have correlations with the development of reproductive organs.

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刘红美,李晓瑜,刘耀光.用RNA干涉对几个水稻WD结构域基因的功能研究[J].华南农业大学学报,2007,28(3):47-51

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  • 最后修改日期:2007-03-14
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