Specific primers were designed to amplify the lpl gene of Phyllotreta striolata (Pslpl gene) by RT-PCR. The cDNA of Pslpl gene was 1 182 bp containing one open reading frame (ORF) encoded 393 amino acids which includes a signal peptide with 13 amino acid residues followed by a putative mature peptide of 380 amino acid residues. The results of the real-time PCR data showed that the Pslpl gene expressed in several tissues of adult beetle, and it was upregulated especially in the head and the midgut. The expression profile was also different between the female and male adults. The expression was higher in male’s reproductive system than that in female’s. However, the expression was higher in other tissues of female. The cDNA of Pslpl gene and its expression profile are the basic knowledge for the further study on lipoate protein ligase and the protein lipoylation of this important pest beetle.