为了研究烤烟致香物质GC/MS指纹图谱在烤烟质量控制中的应用，以南平烤烟为研究对象，将样品根据感官评吸质量档次结果划分为中等、中等+和较好3类，并分别构建了这3类质量档次的标准指纹图谱，计算每一类别样品指纹图谱与标准指纹图谱的相似度.结果表明，相似度值都较大，表明相似性较好.根据指纹图谱相似度确定了每个类别质量档次相似度的阈值，其阈值分别为0.731 2、0.703 6和0.676 7.利用主成分分析的主成分投影图对3个质量档次烤烟样品进行了模式识别，模式识别结果的空间分布较好，区域较明显，表明该质量控制体系有较好的可用性和分辨率.最后利用未知样品对该质量控制体系进行验证，验证结果表明,该体系的准确性较高，误判率较低，适合应用于烤烟质量控制.
In order to study the application of aroma substances GC/MS fingerprint in the quality control of flue-cured tobacco, the Nanping flue-cured tobaccoes were grouped into 3 grades—medium, medium+ and better—according to the sensory evaluation, and the corresponding standard fingerprints of the three grades flue-cured tobacco were established. The fingerprint similarity of each sample with standard fingerprint was calculated. The high values indicated the high similarity, threshold of the three grades were defined according to the similarity of the fingerprint, which were 0.731 2, 0.703 6 and 0.676 7 respectively. Pattern recognization of the three grades flue-cured tobacco was carried out by projection drawing of principal constituent analysis. Pattern recognization results showed the good spatial distribution in the chart, obvious section, which indicated the feasibility and resolution of the quality control system.The unknown samples were used to test the effectiveness of the quality control system. The result indicated that the system had high accuracy, low error rate, and adaptability to the quality control of flue-cured tobacco.
曹建敏,刘帅帅,郭承芳,邱 军,王允白,郭先锋,宁 扬. GC/MS指纹图谱在南平烤烟质量控制中的应用[J].华南农业大学学报,2013,34(1):1-5复制