香蕉枯萎病菌2种细胞壁降解酶酶活性平板检测方法
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

国家自然科学基金(31200112);现代农业产业技术体系建设项目专项(CAR-32-05);华南农业大学校长基金(2007K060)


Plate Assays for Measurement of Two Kinds of Cell Wall Degrading Enzymes Activity in Fusarium oxysporum f. sp. cubense
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    以病原真菌香蕉枯萎病菌4号生理小种为材料,建立了多聚半乳糖醛酸酶和纤维素酶的平板检测方法.多聚半乳糖醛酸酶的最佳产酶和检测条件为:诱导底物为果胶,接种孢子终浓度为1×104 mL-1,培养时间为3 d.37 ℃时检测的多聚半乳糖醛酸酶活性最大;纤维素酶的最佳产酶和检测条件为:诱导底物为羧甲基纤维素,接种孢子终浓度为1×105 mL-1,培养时间为3 d,37 ℃时检测的纤维素酶活性最大.在此基础上,筛选已获得的香蕉枯萎病菌4号生理小种致病力减弱突变体,获得了2株多聚半乳糖醛酸酶和纤维素酶酶活性显著降低的致病突变体.

    Abstract:

    This paper reports a plate assay to detect activities of polygalacturonase and cellulase secreted by Fusarium oxysporum f. sp. cubense (FOC), which is the most destructive pathogen of banana. The optimum cultural conditions for the polygalacturonase production were using pectin as induced substrate to inoculate with 1×10 4 mL-1 conidia of FOC and after being cultured for 3 days. The optimal temperature of polygalacturonase activity was 37 ℃. The optimum cultural conditions for cellulase production were using carboxymethyl cellulose (CMC) as induced substrate to inoculate with 1×10 5 mL-1 conidia of FOC in inducing medium after being cultured for 3 days. The optimal temperature of cellulase activity was 37 ℃. Based on the method, two mutants with impaired polygalacturonase and cellulose activity were attained through screening virulence-reduced mutants of FOC.

    参考文献
    相似文献
    引证文献
引用本文

施金秀,林先丰,姜乃月,凌金锋,李敏慧,姜子德.香蕉枯萎病菌2种细胞壁降解酶酶活性平板检测方法[J].华南农业大学学报,2013,34(3):340-344

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2012-11-12
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2013-06-27