酶活性、丙二醛含量变化与苦瓜抗枯萎病的关系
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广东现代农业产业技术体系建设专项资金;广东省科技计划项目(2012A020100006,2012B020408001)


The Relationship Between Malonaldehyde Content, Enzymatic Changes and the Resistance of Bitter Gourd to Fusarium Wilt
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    摘要:

    以苦瓜枯萎病高感自交系‘472113’、高抗自交系‘0417’为材料,探讨了苦瓜幼苗接种枯萎病菌后的生理生化变化与抗病性的关系.结果表明,多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、过氧化氢酶(CAT)、几丁质酶(CHT)、β-1, 3-葡聚糖酶(GUN)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性与抗病性呈正相关,而丙二醛(MDA)含量与抗病性呈负相关.初步认为可用PPO、PAL、CAT、CHT、GUN、POD和SOD活性、MDA含量的峰值大小作为反映苗期苦瓜材料枯萎病抗性的生理生化指标,其中首选PPO活性,其次是CHT、GUN活性和MDA含量,因为它们的变化与苦瓜枯萎病抗性关系更为密切.

    Abstract:

    By using the sensitive (‘472113’) and resistant (‘0417’) inbreed lines to bitter gourd fusarium wilt as materials, the physiological and biochemical changes of Momordica charantia seedling were studied after inoculation with pathogen. The results indicated that activities of polyphenoloxidase (PPO), phenylalanine ammonialyase(PAL), catalase (CAT), chitinase (CHT), β-1, 3-glucanase (GUN), peroxidase (POD) and superoxide dismutase (SOD) had a positive correlation with disease resistance. However, malonaldehyde (MDA) content had a negative correlation with disease resistance. In conclusion, the peak value of PPO, PAL, CAT, CHT, GUN, POD, SOD activities and MDA content could be taken as the indicator for bitter gourd fusarium wilt resistance, and PPO activity was the best followed by CHI, GUN activity and MDA content, due to their much closer correlation with bitter gourd Fusarium wilt resistance.

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赵秀娟,唐 鑫,程蛟文,李卫鹏,谭 澍,胡开林.酶活性、丙二醛含量变化与苦瓜抗枯萎病的关系[J].华南农业大学学报,2013,34(3):372-377

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  • 收稿日期:2012-10-29
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  • 在线发布日期: 2013-06-27