【目的】为改良玉米雄穗性状.【方法】以雄穗一级分枝数有显著差异的超甜玉米自交系 T4 和 T19 为亲本，构建了包含232个单株的 F2 群体，考察雄穗一级分枝数，利用复合区间作图法进行 QTL 定位.【结果和结论】结果获得一张包含77个 SSR 标记的遗传连锁图谱，全长868.7 cM，标记平均间距为11.28 cM，共检测到4个与超甜玉米雄穗一级分枝数相关的 QTL 位点，分别位于玉米第4、7、8染色体上，可解释5.08%~17.71%的表型变异.主效 QTL 位点 qTBN-4 位于第8染色体，可解释17.71%的表型变异.这些 QTLs 将为雄穗一级分枝数的分子标记辅助选择提供依据.
【Objective】To improve the tassel of maize.【Method】In this study, a cross between sweet corns inbred T4 and T19, which were significantly different in tassel primary branch number, was designed and QTLs for tassel primary branch number were identified in a whole genome with the methods of multiple interval mapping (CIM) based on 232 F2 individuals. 【Result and conclusion】 A genetic linkage map composing of 77 simple sequence repeat (SSR) markers covering 868.7 cM with an average interval of 11.28 cM was constructed. Four QTLs for tassel primary branch number in chromosome 4, 7, 8 were detected, accounting for 5.08%-17.71% of phenotypic variance. The main QTLs were located on chromosome 8 which accounted for 17.71% of the total phenotypic variance. These QTLs provide the basis of marker-assisted selection for tassel primary branch number.
张姿丽,蒋 锋,刘鹏飞,陈青春,张 媛,王晓明.甜玉米雄穗一级分枝数 QTL 定位[J].华南农业大学学报,2014,35(1):110-113复制