含 2 - 氨甲基苯并咪唑铜(Ⅱ)配合物的合成、表征、抑菌活性及与 DNA 作用的研究
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

华南农业大学“211工程”三期重点建设项目(2009B010100001)


Syntheses,characteristics,bacteriostatic activities and their interaction with DNA of copper(Ⅱ) complexes containing 2-aminomethylbenzimidazole
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    【目的】为了设计和寻找DNA的特异性识别剂和断裂剂,合成了2 - 氨甲基苯并咪唑铜(Ⅱ)配合物:[Cu(AMB)2Cl]Cl·4H2O(配合物1)和[Cu(AMB)(phen)Cl]Cl·2H2O(配合物2)(AMB=2 - 氨甲基苯并咪唑,phen=1,10 - 邻菲咯啉).【方法】通过元素分析、IR、UV和摩尔电导率对配合物进行了表征.用二倍稀释法测试了配合物对大肠埃希菌 Escherichia coil(G-)、沙门杆菌 Salmonella typhi(G-)、金黄色葡萄球菌 Staphylococcus aureus(G+)和枯草芽孢杆菌 Bacillus subtilis(G+)的最小抑菌浓度(MIC).采用电子吸收光谱、荧光光谱、相对黏度及琼脂糖凝胶电泳法测试了2个配合物与 ct-DNA 的结合作用.【结果和结论】配合物1以非插入方式、配合物2以插入方式与 ct-DNA 作用;在 VC 存在下,2个配合物均通过·OH氧化机理切割 pBR322 DNA.2个配合物结合 ct-DNA 和切割 pBR322 DNA 的能力强弱均为:配合物2> 配合物1.

    Abstract:

    【Objective】In order to design and find specific recognition and cleavage agent of DNA, two copper(Ⅱ) complexes,[Cu(AMB)2Cl]Cl·4H2O(complex 1)and[Cu(AMB)(phen)Cl]Cl·2H2O(complex 2)(AMB = 2-aminomethylbenzimidazole, phen=1,10-phenanthroline), were synthesized. 【Method】The two complexes were characterized by elemental analysis, IR, UV and molar conductivity. The complex 1 and complex 2 were assayed against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria(Escherichia coil and Salmonella typhi) by a double dilution method, and the interaction with ct-DNA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and agarose gel electrophoresis.【Result and conclusion】The complex 2 could interact with DNA by non-intercalation, while complex 1 by non-intercalation, and they cleave pBR322 DNA assisted by vitamin C involving the hydroxyl radical oxidation mechanism. The order of combination ability with ct-DNA and cleaving pBR322 DNA of the two complexes is complex 2>complex 1, which is in accordance with the bacteriostatic activity.

    参考文献
    相似文献
    引证文献
引用本文

王 璐,段冉冉,赵 盼,周晓华.含 2 - 氨甲基苯并咪唑铜(Ⅱ)配合物的合成、表征、抑菌活性及与 DNA 作用的研究[J].华南农业大学学报,2014,35(2):90-95

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2013-02-21
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2014-02-28