鸡嗉囊中淋巴细胞发育的研究
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国家自然科学基金(30901065); 吉林省科技发展计划项目(201205059); “十二五”吉林省教育厅科学技术研究项目(2011431)


A study on development of lymphocytes in the chicken crop
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    【目的】研究鸡出壳后不同日龄嗉囊中 T 淋巴细胞及其亚群和B淋巴细胞的定位分布及数量变化规律等一系列的发育过程.【方法】采用单克隆抗体,通过免疫组织化学方法,观察并统计各取材日龄相应细胞的分布和数量.【结果和结论】1日龄时,CD3+、CD4+ 和CD8+ 细胞即已出现,Bu-1b+ 细胞于出壳后4日龄时出现.定位分布上,CD3+ 细胞主要分布于黏膜上皮基底层及固有层中,7日龄首次出现以CD3+ 细胞为中心的淋巴聚集物;CD4+ 细胞主要分布于固有层中;CD8+ 细胞最初主要分布在黏膜固有层中,随日龄增长逐渐向上皮方向突入,最终形成含有CD8+ 细胞浸润的淋巴上皮;Bu-1b+ 细胞主要分布于黏膜固有层中,特别是在小血管周围.于35日龄观察到以Bu-1b+ 细胞为中心的生发中心.在数量变化上,CD3+、CD4+、CD8+ 细胞数量整体呈逐渐增加趋势,部分阳性细胞数量在14和21日龄时稍有下降,35~56日龄时阳性细胞数量缓慢增加,基本达到成熟水平.Bu-1b+ 细胞数量随着日龄的增加持续增多,且后期的增长速率大于 T 细胞的增长速率.结果表明,嗉囊作为鸡消化系统的一部分,具有由淋巴细胞和淋巴聚集物构成的免疫防线,为肠相关淋巴组织中的一个重要组成部分.

    Abstract:

    【Objective】To study the appearance, distribution and quantity variation of immunocytes—T lymphocytes and its subpopulations and B lymphocytes in different periods, analyzing the developmental process of immunocytes in the chicken crop.【Method】The mono-antibodies and immunohistochemistry was used to observe the distribution and count the number of the relevant cell in every age. 【Result and conclusion】The results showed that CD3+, CD4+ and CD8+ cells appeared in the first day after post-hatching. Bu-1b+ cells were observed in the 4rh day. CD3+ cells mainly distributed in the epithelium and lamina propria, and the lymphoid aggregates covered by CD3+ cells were first observed in the 7th day after post-hatching. CD4+ cells were located in the lamina propria. At first, CD8+ cells mainly distributed in the lamina propria of the mucosa. With the age increasing, CD8+ cells slightly migrated and infiltrated to the epithelium of the mucosa,which were transformed to the lymphoepithelium. Bu-1b+ cells mainly distributed in the lamina propria, especially around the vessels. The germinal center which was covered by the Bu-1a+ cells was first observed in the 35th day after post-hatching. The amount of CD3+, CD4+, and CD8+ cells kept increasing with the age; the amount of parts of positive cells decreased in the 14th and 21th day; it slightly increased after this date and reached mature level around the 56th day. Bu-1b+ cells continuously kept increasing with age, its rising rate was higher than T lymphocytes during the later period. The research showed that as a part of the digest system in the chicken, the crop posses ses the mucosal immunity which consists of the lymphoid aggregates and the lymphocytes.The crop is an important part of gut-associated lymphoid tissue(GALT).

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李磊,张英楠,杨树宝,冯小刚,刘莹,董红艳,马金萍,栾维民.鸡嗉囊中淋巴细胞发育的研究[J].华南农业大学学报,2014,35(3):24-29

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  • 收稿日期:2013-09-11
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  • 在线发布日期: 2014-04-15