番茄 SSR 标记在茄子及其他茄科蔬菜作物上的通用性分析
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科技部国际合作项目(2010DFA32190) ;国家自然科学基金(31171957,30771472);广东省科技厅国际合作项目(2009B050100001,2011B050100013);广州市科技支撑计划项目(2010Z1-E381)


Transferability of tomato SSR markers to eggplants and other Solanaceous vegetables
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    【目的】利用已完成测序的番茄基因组发展大量的SSR标记,并将这些标记转移到茄子及其他茄科作物上,节省开发SSR标记的成本.【方法】本研究利用近缘物种转移法分析了番茄SSR标记在茄子及其他茄科作物上的通用性情况.【结果和结论】1 046对番茄SSR引物中有887对能在茄子基因组DNA上扩增出产物,425对引物扩增出的带型在番茄与茄子间相似程度高,标记的通用率为40.6%;EST-SSR比基因组SSR的通用性更好,前者通用率为54.5%,后者为38.9%;414个通用SSR标记被电子定位到番茄染色体上,不同染色体来源的标记通用率明显不同;93对引物在2份用于遗传图谱构建的栽培茄子亲本间表现出多态性;获得的425对通用引物在马铃薯、辣椒、枸杞上通用率分别为96.2%、78.1%、54.1%.

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    【Objective】Tomato genome was sequenced and a large number of SSR markers could be obtained. Cross-species amplification of tomato SSR markers will be an economic method to add up the number of robust SSR markers in eggplant and Solanaceous vegetables. 【Method】 This study tested a large set of tomato SSR markers on eggplant and other Solanaceous vegetables by close relative species transfer method. 【Result and conclusion】 887 out of 1046 tested tomato SSR markers could successfully amplify on eggplants, but only 425 produced very similar bands on tomatoes and eggplants, giving a transfer rate of 40.6%. EST-SSR markers were found to be more transferable than genomic SSR markers (54.5 and 38.9% respectively). 414 transferable SSR markers were mapped electronically onto tomato genome and their distribution on individual chromosomes highly varied. 93 SSR markers showed polymorphism on two eggplant parents used for genetic map construction. When testing 425 cross-species SSR markers on potatoes, peppers and lycium, the transferability rates were 96.2%, 78.1% and 54.1%, respectively.

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汪国平,牛玉,汪文毅,乐素菊,林鉴荣.番茄 SSR 标记在茄子及其他茄科蔬菜作物上的通用性分析[J].华南农业大学学报,2014,35(4):56-60

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  • 收稿日期:2013-04-10
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  • 在线发布日期: 2014-06-17