广东和江西省柑橘溃疡病菌的遗传多样性分析
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公益性行业(农业)科研专项经费项目子课题 (201003067-01-7)


Genetic diversities of Xanthomonas citri subsp.citri in Guangdong and Jiangxi Provinces
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    【目的】明确来自广东和江西两省柑橘溃疡病菌菌株之间的分子水平差异.【方法】使用ERIC引物,通过REP-PCR技术,对来自我国广东和江西省12个市(县)12个柑橘品种的71个柑橘溃疡病菌株进行遗传多样性研究.根据DNA指纹特征,并用NTSYS软件聚类分析.【结果和结论】71个菌株在相似值为0.64时,可以分为A、B两簇,其中来自江西赣州6个市县的所有溃疡病菌株全部聚在A簇,而来自广东有50%的菌株聚在B簇.取相似值为0.88时,A、B簇又各自分为6个遗传组.广东和江西两省柑橘溃疡病菌存在明显的遗传多样性,不同地理来源和不同柑橘品种之间的溃疡病细菌多样性差异明显.

    Abstract:

    【Objective】 Citrus canker caused by Xanthomonas citri subsp. citri (Xcci) is one of the most important plant diseases.The aim of the experiment was to study the genetic diversities of Xac in Guangdong and Jiangxi Provinces.【Method】The bacterial strains which were collected from 12 cities (counties) of Guangdong and Jiangxi Provinces on 12 varieties were investigated with ERIC-PCR.Dendrograms were generated by NTSYS software according to fingerprint features.【Result and conclusion】 The results indicated that 71 Xac strains could be clustered division A and B.All the strains from Jiangxi Province were distributed on division A, while half of the strains from Guangdong Province were distributed on division B. Both division A and B could be seperated into 6 groups at a level of 88% similarity respectively. There were significant genetic diversities of Xac strains from Guangdong and Jiangxi Provinces. It showed that the diversity differences of strains from different geographical origins or different varieties were significant.

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魏楚丹,丁钿,叶淦,刘琼光.广东和江西省柑橘溃疡病菌的遗传多样性分析[J].华南农业大学学报,2014,35(4):71-76

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  • 收稿日期:2013-10-20
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  • 在线发布日期: 2014-06-17