【目的】提高‘热研4号’王草 Pennisetum purpureum × P. americanum cv. Reyan No.4 生产乙醇的转化率.【方法】采用石灰进行预处理，对时间、温度、石灰用量、固液比4个因素进行单因素和正交优化试验分析.【结果和结论】最优预处理条件为:时间3 h,温度125 ℃,原料的石灰用量0.15 g·g-1，固液比(m∶V)1∶12（g·mL-1）.此时‘热研4号’王草的干基失质量率、木质素移除率、半纤维素移除率和纤维素移除率分别为28.1%、65.4%、37.5%和6.1%.预处理的‘热研4号’王草经酶解后，半纤维素、纤维素和总酶解得率分别为43.5%、85.7%和55.2%，比未经处理的酶解得率分别提高了18.1、4.5和4.4倍.此外，影响酶解得率的主要因素是预处理时间和温度，其次是石灰用量和固液比.
【Objective】 The pretreatment of Pennisetum purpureum × P. americanum cv. Reyan No.4 was studied with lime to improve the conversion rate for ethanol production. 【Method】 The effects of pretreatment time, temperature, the lime dosage, and solid-to-liquid ratio as well as the orthogonal optimization test on the composition and the following enzymatic hydrolysis of ‘Reyan No.4’ were investigated.【Result and conclusion】 The optimal pretreatment conditions were as follows: pretreatment time 3 h, temperature 125 ℃, solid-to-liquid ratio 1∶12 （g·mL-1） and the lime dosage of material 0.15 g·g-1. Under the optimal condition, dry mass loss was 28.1% in lime pretreatment, and the removal rates of lignin, hemicellulose and cellulose were 65.4%, 37.5%, 6.1%, respectively. The yields of hemicellulose, cellulose and total residue were 45.5%, 85.7%, 55.2%, respectively; in the following enzymatic hydrolysis, they increased 18.1 times, 4.5 times and 4.4 times, respectively, compared with those of untreated ‘Reyan No.4’. The primary factors influencing enzymatic hydrolysis yield were temperature and time, followed by lime dosage and solid-to-liquid ratio.