1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位
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国家自然科学基金(31170731,31200611);上海市科学技术委员会资助项目(10DZ2271800);吉林省科技发展计划项目(20130206012NY)


A genetic analysis and gene mapping of a new translucent and shrunken endosperm mutant in maize
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    【目的】解析半透明皱缩玉米胚乳形成机制.【方法】以纯合玉米突变体M-E-479为供试材料,通过对M-E-479籽粒进行表型鉴定、遗传分析、主要成分测定及扫描电镜观察,构建定位群体,利用图位克隆技术对突变基因进行遗传定位.【结果和结论】M-E-479突变体胚乳中脂肪含量增加,蛋白质和淀粉含量下降,淀粉粒的大小、形状、排列空间发生改变;用F2群体将突变基因定位在10号染色体SSR标记bnlg1074与umc1506之间,两标记的遗传距离约为3.6 cM,两标记的物理距离约为2.2 Mb.可见M-E-479是一个新的玉米半透明皱缩胚乳突变体,突变性状是由隐性单基因控制.

    Abstract:

    【Objective】 To analyze the formation mechanism of translucent and shrunken maize endosperm.【Method】 The mutant M-E-479 of homozygous maize was used as experimental materials in our study. Through phenotypic identification, genetic analysis, main ingredients measurement and scanning electron microscopy, mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene.【Result and conclusion】 The result showed that the amount of fat increased, while the protein and starch decreased, and the starch grains changed in size, shape and space configuration in the mutant endosperm of M-E-479. The mutant gene is located between SSR markers bnlg1074 and umc1506 of chromosome 10 by using F2 population. Meanwhile, the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb. The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant, and its mutant character may be controlled by a recessive single gene.

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崔喜艳,胡广宇,孙小杰,佟珊珊,陈众峰,刘相国.1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位[J].华南农业大学学报,2014,35(5):31-35

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  • 收稿日期:2013-09-21
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  • 在线发布日期: 2014-07-29