广东省不同区域菜园土壤微生物群落功能多样性比较
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广东省科技计划项目(2012A020100004)


An comparative study on functional diversity of soil microbial community in vegetable garden soils from different regions of Guangdong Province
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    【目的】了解广东省不同区域菜园土壤微生物碳代谢功能群落结构的特点与差异.【方法】采用 Biolog 生态微平板方法分析广东的粤北、粤东、粤西和粤中4个不同区域菜园土壤微生物碳代谢群落结构.【结果和结论】4个不同区域的土壤微生物平均颜色变化率(AWCD )、Shannon 指数、Simpson 指数、McIntosh 指数变化趋势均不同,反映微生物活性的AWCD表现为:粤北>粤东>粤西>粤中,表明不同区域菜园土壤微生物在碳源利用能力、微生物丰度等方面存在差异. 4个区域土壤微生物对6类31种碳源的利用程度存在差异;主成分分析显示,4个区域土壤微生物代谢基质主成分1的贡献率为54.76%,主成分2为13.25%;主成分1 载荷0.18 以上的基质有22 种,主成分2 有15 种;碳源在主成分分离中起主要贡献作用的是氨基酸类、羧酸类、酚类和碳水化合物类碳源.表明不同区域菜园土壤微生物群落碳源利用模式及代谢功能不同,即土壤微生物群落功能多样性有差异.

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    【Objective】To understand the characteristics and differences among the carbon catabolic diversities of vegetable garden soil microbes from the different regions of Guangdong Province. 【Method】The community structures of vegetable garden soil microbes in the northern, eastern,western and central regions of Guangdong Province(NR,ER,WR and CR) were analyzed using the Biolog Eco-Plate Method.【Result and conclusion】There were different changing tendencies of the Average Well Color Development (AWCD), Shannon Index, Simpson Index and McIntosh Index in the four regions. The AWCD value, which directly reflected the microbial activities, was in the order of NR>ER>WR>CR, demonstrating the differences among the uses of carbon sources and abundance in vegetable garden soil microbes from the different regions. There were differences among utilization degrees of the 31 types, 6 classes of carbon sources by the vegetable garden soil microbes from the four regions. Through principal component analysis, it showed that the contribution rate of the PC1 of the metabolism substrates of vegetable garden soil microbes in the four regions was 54.76%, PC2 was 13.25%, and there were 22 types of metabolism substrates in PC1 with over 0.18 of load,but only 15 types in PC2.Amino acids, carboxylic acids, phenols and carbohydrates were the four main carbon sources separating the two principal component factors. In conclusion, the carbon source utilization modes and metabolic functions of the microbes among the vegetable garden soils from the four regions differ from each other, that is, there are different functional diversities of microbial community among the vegetable garden soils from the four regions under investigation.

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赵兰凤,刘小锋,官利兰,程根,张新明.广东省不同区域菜园土壤微生物群落功能多样性比较[J].华南农业大学学报,2015,36(1):54-59

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  • 收稿日期:2014-01-26
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  • 在线发布日期: 2015-01-06