【Objective】Isolation and identification of resistance gene analogs (RGAs) of Lagenaria siceraria would lay the foundation for a further cloning of disease resistance genes and marker-assisted selection (MAS) of resistance breeding. 【Method】According to the conserved domains of nucleotide binding site and leucine rich repeat (NBS-LRR) type of disease-resistance genes in most known plants, degenerate primers were designed and synthesized to isolate resistance gene analogs from genomic DNA of bottle gourd resistant variety “Dazihu”, with length variation, conservative domain, homology alignment and phylogenetics analyzed by various bioinformatics softwares. 【Result and conclusion】Twenty three RGAs were obtained and named as HNB1-HNB23, and the GenBank accession numbers were KJ908192-KJ908214. Sequences analyses and alignment results indicated that the full-length of RGAs varied from 242 nt to 261 nt, and the deduced amino acids sequences contained typical conserved domains of NBS R genes, such as P-loop and Kinase-2a. Eighteen sequences had continuous open reading frames (ORFs). These RGAs showed a great homologous differences with the similarity ranging from 41.5% to 98.8%, and the amino acid sequence similarity varied from 21.5% to 100.0%. At the nucleotide level, the sequence identity of 23 RGAs ranged from 40% to 100% with the cloned NBS R genes from other plants, especially cucumber, wax gourd, luffa and calabash. The result of clustering analyses showed that all RGAs were divided into 5 groups. These RGAs were ranked into non-TIR-NBS-LRR type by homology and evolution analyses, which was consistent with the classification result based on multiple alignment of deduced amino acid sequences.