1株桑根腐病拮抗细菌的分离鉴定及田间防治效果
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

国家自然科学基金(31270076);广西科学研究与技术开发计划项目(桂科重14121002-4-3);南宁市科学研究与技术开发计划项目(20122065)


Isolation, identification and field control capability of an antagonistic bacterium strain against mulberry root rot
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    【目的】筛选出对桑根腐病菌可可毛色二孢 Lasiodiplodia theobromae 有显著拮抗作用的细菌,并测定其田间防治效果。【方法】采用平板稀释法从健康桑树根际土壤中分离细菌;采用平板对峙法和孢子萌发法筛选出对靶标菌株有显著拮抗作用的菌株。通过形态观察、生理生化特征测定、gyrAgyrB基因序列鉴定拮抗细菌菌株。设置田间小区试验,测定拮抗菌株在自然条件下的生防效果。【结果】从桑树根际土壤中分离出8株对L. theobromae有抑制作用的细菌,其中菌株YZ14-3的抑制效果最好。该菌株及其培养液对L. theobromae菌丝生长的抑制率分别为73.3%和55.6%,挑取抑菌圈上的L. theobromae菌丝镜检发现,菌丝畸形膨大;L. theobromae分生孢子在YZ14-3的培养液中不能萌发,且分生孢子因细胞壁降解而解体;经形态观察、生理生化特征测定和基于gyrAgyrB基因序列BLAST分析结果构建的系统发育树,YZ14-3被鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens;大田防治试验结果表明,YZ14-3的生防效果达67.94%。【结论】B. amyloliquefaciens菌株YZ14-3对L. theobromae的抑制效果较显著,可应用于L. theobromae所致病害的生物防治。

    Abstract:

    【Objective】 To isolate bacterium strains that have strongly inhibitory effects against Lasiodiplodia theobromae, the causal agent of mulberry root rot, and test its field control efficiency.【Method】Bacterium strains were isolated from rhizosphere soil of healthy mulberry by plate dilution method.Strains with remarkable antagonist activities against target pathogen were screened by dual culture and spore-germination techniques. The antagonistic bacteria were identified based on morphological, physiological and biochemical characteristics as well as their gyrA and gyrB gene sequences. Field plot experiments were set up to determine the biocontrol effects of antagonistic bacteria under natural conditions.【Result】Eight bacterium strains with inhibitory effects against L. theobromae were isolated, and YZ14-3 was the most effective among them. The inhibition rate of YZ14-3 and its culture extracts were 73.3% and 55.6%, respectively. The mycelia of L. theobromae in the inhibition zone were enlarged and malformed; the conidia could not germinate and disintegrated due to cell wall degradation in the culture extracts of YZ14-3. YZ14-3 was identified as Bacillus amyloliquefaciens using its morphological, physiological and biochemical characteristics as well as the phylogenetic tree based on the BLAST results with gyrA and gyrB gene sequences. Field tests showed that the biocontrol efficiency of YZ14-3 was 67.94%.【Conclusion】The B. amyloliquefaciens strain YZ14-3 has remarkable inhibitory effect against L. theobromae, and therefore it can be used for biocontrol of diseases caused by L. theobromae.

    参考文献
    相似文献
    引证文献
引用本文

谢红辉,韦继光,周颖,熊英.1株桑根腐病拮抗细菌的分离鉴定及田间防治效果[J].华南农业大学学报,2016,37(2):59-64

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2015-07-03
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2016-02-02