硝磺草酮降解菌HZ-2制剂固定化载体材料性能的比较
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广东省科技计划项目(2014B020206003)


Pexformance comparison of different carrier materials for immobilization of mesotrione-degrading strain HZ-2
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    【目的】研究改性蔗渣MSB、MSB-Mo、MSB-Cl对硝磺草酮降解菌HZ-2的降解能力的影响。【方法】以这3种改性蔗渣和活性炭为固定化载体,对固定化后菌制剂的降解性能进行研究。【结果】降解菌制剂培养48 h后,D600 nm 均较高,表明MSB、MSB-Mo、MSB-Cl及活性炭均具备较强的固菌能力;培养3 d 后,以改性蔗渣为载体的固菌制剂对LB培养基中硝磺草酮的降解率高于85%,显著优于活性炭,其中改性蔗渣MSB-Mo效果突出,降解率达96.35%。添加葡萄糖辅料7 d后,这3种改性蔗渣对土壤中硝磺草酮的降解率均高于99%。【结论】改性蔗渣MSB-Mo是固定降解菌株的最佳新型生物载体材料。

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    【Objective】 To evaluate the effect of modified bagasse (MSB, MSB-Mo and MSB-CI) on the immobilization of mesotrione-degrading bacteria strain HZ-2.【Method】Three types of modified bagasse and activated carbon were selected as the carrier candidates for the bacterial immobilization,and the degradation abilities of immobilized composites were examined.【Result】After 48 hours culture, the high D600 nm values indicated that modified bagasse(MSB, MSB-Mo and MSB-CI) and activated carbon had strong abilities to immobilize bacteria. More than 85% of mesotrione in LB medium was degraded using modified bagasse as a carrier after 3 days of culture, which was higher than the results of activated carbon. MSB-Mo composite had the best degradation performance with a degradation rate of 96.35%.Moreover,mesotrione in soil was degraded over 99% after 7 days by adding glucose as support nutrient to the modified bagasse. 【Conclusion】 Modified bagassse MSB-Mo is the most available carrier material, which demonstrates the highest potential of being a new biological carrier material for immobilization of mesotrione-degrading bacteria strain.

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张晶晶,杨孟然,刘婕,蒋刚彪,钟国华.硝磺草酮降解菌HZ-2制剂固定化载体材料性能的比较[J].华南农业大学学报,2016,37(3):86-89

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  • 收稿日期:2015-07-05
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  • 在线发布日期: 2016-05-05