【目的】测定球孢白僵菌 Beauveria bassiana 对亚洲玉米螟Ostrinia furnacalis 血细胞系SYSU-OfHe-C的影响，为促进球孢白僵菌在防治亚洲玉米螟中的应用提供理论依据。【方法】采用MTT和CCK-8法测定球孢白僵菌对亚洲玉米螟血细胞系SYSU-OfHe-C的毒力，通过光学显微镜与扫描电镜观察球孢白僵菌生长及其对SYSU-OfHe-C细胞的影响。【结果】球孢白僵菌分生孢子处理SYSU-OfHe-C细胞24 h后，MTT法的IC50值为2.8×105 mL-1，CCK-8法的IC50值为1.4×105 mL-1。光学显微镜实时监测发现，在SYSU-OfHe-C细胞对数期接种球孢白僵菌浓度为3.5×104 mL-1时，球孢白僵菌具有明显竞争力，未发现囊胞化与吞噬等细胞免疫现象；处理10 h后，球孢白僵菌分生孢子开始萌发长出菌丝，而SYSU-OfHe-C细胞减少且开始变形；处理24 h，球孢白僵菌几乎长满整个视野；SYSU-OfHe-C细胞从近似圆形变长或者变为不规则形状，出现聚集、破碎、胞内空泡、瘤状突起，以及被白僵菌菌丝穿透等现象。【结论】球孢白僵菌可改变亚洲玉米螟血细胞系SYSU-OfHe-C的细胞形态，但不能使其发生细胞免疫现象。
【Objective】To test the effect of Beauveria bassiana on SYSU-OfHe-C, a cell line from hemocytes of Ostrinia furnacalis. To provide a theory basis for application of B.bassiana in the prevention and treatment of O. furnacalis.【Method】MTT and CCK-8 were employed to test the virulence of B.bassiana on SYSU-OfHe-C. Optical microscope and scanning electron microscope were used to observe the growth of B.bassiana and its influence on the morphology of SYSU-OfHe-C cells. 【Result】The IC50 values of B.bassiana against SYSU-OfHe-C cells at 24 hours after treatment were recorded as 2.8×105 and 1.4×105 mL-1 respectively by MTT and CCK-8. Furthermore, under real-time monitoring with optical microscope, encapsulization and phagocytosis were not found and B.bassiana was highly competitive when the conidia of B. bassiana were inoculated in the culture of SYSU-OfHe-C cells at logarithmic stage reaching a final concentration of 3.5×104 mL-1 for B.bassiana. B.bassiana started to germinate at 10 hours after treatment, and meanwhile, SYSU-OfHe-C cells started to reduce in number and change in morphology. At 24 hours after treatment, B.bassiana grew the entire visual field. The nearly round SYSU-OfHe-C cells had elongated or turned into irregular shape. Aggregation, fragmentation, cavitation, protuberance and penetration of SYSU-OfHe-C cells caused by B.bassiana mycelia were observed.【Conclusion】B.bassiana can change cellular morphology of hemocytes of O. furnacalis, but cannot induce its immune responses.