【Objective】To explore the influence of G418 treatment of donor cells on the developmental efficiency of somatic cell nuclear transfer (SCNT) embryos.【Method】Porcine adult fibroblasts were collected after six days of G418 treatment. Expression levels of antioxidation and apoptosis-related genes were detected by quantitative RT-PCR before and after G418 treatment. The DNA methylation levels of LINE-1 repetitive sequences and microsatellites were analyzed using bisulfites sequencing analysis, and the in vitrodevelopmental rates of SCNT embryos were investigated.【Result】G418 treatment of donor cells caused significant (P<0.05) changes in expression levels of genes related to antioxidation and apoptosis, but did not significantly(P>0.05) change DNA methylation levels. SCNT embryos cloned from G418-treated donor cells exhibited a significantly (P<0.05) lower in vitro developmental rates compared with the control group.【Conclusion】G418 treatment of donor cell may inhibit the development of SCNT embryo.