池杉叶片和球果挥发油化学成分分析及抗细菌活性
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广东省林业科技创新项目(2015KJCH043); 广东省普通高校青年创新人才项目(2014KQNCX034)


Chemical compositions and antibacterial activities of volatile oils from Taxodium ascendens leaves and cones
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    【目的】分析和鉴定池杉Taxodium ascendens叶片和球果挥发油的化学组成,测定挥发油对7种供试细菌的抑制活性。【方法】采用水蒸气蒸馏法分别提取池杉叶片和球果中的挥发油,通过GC-MS对提取得到的挥发油进行化学成分分析,采用滤纸片扩散法测定挥发油对供试细菌的抑制活性。【结果】池杉叶片和球果中挥发油的得率分别为0.211%和0.657%。从池杉叶片挥发油中鉴定出21种成分,占挥发性成分总量的90.410%,主要成分为(1R)-α-蒎烯(70.149%)、α-松油醇(7.072%)、4-蒈烯(2.025%)和β -蒎烯(2.012%);从池杉球果挥发油中鉴定出13种成分,占挥发性成分总量的95.285%,主要成分为(1R)-α-蒎烯(78.609%)、铁锈罗汉柏醇(4.276%)、4-蒈烯(3.355%)、柠檬烯(2.324%)和β -萜品烯(2.179%)。池杉叶片和球果挥发油中相同的成分只有4种,分别是(1R)-α-蒎烯、4-蒈烯、柠檬烯和冰片。池杉球果挥发油对根癌农杆菌Agrobacterium tumefaciens的抑制活性最强,抑菌圈直径为(34.5 ± 2.3)mm,而池杉叶片挥发油对溶血葡萄球菌Staphylococcus haemolyticus的抑制活性最强,抑菌圈直径为(16.0 ± 1.2)mm。【结论】池杉球果中挥发油的含量高于其叶片,二者的主要成分均为(1R)-α -蒎烯,池杉球果挥发油的抗细菌活性明显强于叶片挥发油。

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    【Objective】To analyze and identify the chemical compositions of volatile oils extracted from Taxodium ascendens leaves and cones, and test the inhibition activities against seven different bacteria.【Method】The volatile oils were extracted by hydro-distillation from the leaves and cones of Taxodium ascendens and the chemical compositions of the essential oils were analyzed by GC-MS. The inhibitory activities against bacteria were tested using the filter paper diffusion method. 【Result】The yield rates for the essential oils of fresh leaves and cones were 0.211% (w) and 0.657% (w), respectively. Totally 21 components were identified from the volatile oil of leaves, which accounted for 90.410% of the total volatile content. (1R)-α-pinene (70.149%), (-)-α-terpineol (7.072%), 4-carene (2.025%) and β-pinene(2.012%) were the major compounds in the volatile oil of leaves. Totally 13 components were identified from the volatile oil of cones, which accounted for 95.285% of the total volatile content. (1R)-α-pinene (78.609%), ferruginol (4.276%), 4-carene (3.355%), limonene (2.324%) and β-terpinene (2.179%) were the major compounds in the volatile oil of cones. There were only four common components in the volatile oils of both leaves and cones including (1R)-α-pinene, 4-carene, limonene and borneol. The volatile oil of cones showed the strongest inhibitory activity against Agrobacterium tumefaciens and the inhibition zone diameter was (34.5±2.3) mm, while the volatile oil of leaves showed the strongest inhibitory activity against Staphylococcus haemolyticus and the inhibition zone diameter was (16.0±1.2) mm.【Conclusion】The content of volatile oil in T. ascendens cones is higher than that in leaves, and (1R)-α-pinene is the main ingredient in both. The volatile oil of cones has higher antibacterial activity than that of leaves.

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单体江,唐祥佑,刘易,王伟,陈璇,段志豪,伍慧雄,王军.池杉叶片和球果挥发油化学成分分析及抗细菌活性[J].华南农业大学学报,2016,37(5):72-76

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  • 收稿日期:2016-01-17
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  • 在线发布日期: 2016-07-25