球孢白僵菌与玫烟色棒束孢制剂对柑橘木虱的防治
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广东省科技计划 ( 2015A020209140); 广州市科技计划(201509010023); 广州市产学研协同创新重大专项对外合作项目(201604030029); 广东省普通高校省级重大科研项目(2014-16)


The biocontrol effects of Beauveria bassiana and Isaria fumosorosea on Asian citrus psyllid
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    【目的】研究2株高致病力菌株球孢白僵菌Beauveria bassiana和玫烟色棒束孢Isaria fumosorosea菌株对柑橘木虱Diaphorina citri不同虫态的致病力及田间防控效果。【方法】球孢白僵菌和玫烟色棒束孢孢子稀释成1×104、1×105、1×106、1×107、1×108 mL-1,室内喷施法研究其对木虱低龄、高龄若虫与成虫的侵染致死效果;在笼罩条件下研究其在半田间条件下对柑橘木虱成虫种群的控制作用。【结果】 2种真菌制剂对柑橘木虱低龄若虫的致病力高于高龄若虫。7 d后球孢白僵菌和玫烟色棒束孢对柑橘木虱高龄若虫的LC50值分别为3.6×104和5.2×104 mL-1,对低龄若虫的LC50值分别为3.5×104和4.2×104 mL-1,而对柑橘木虱成虫的LC50值分别为1.4×105和1.6×105mL-1。在半田间条件下,球孢白僵菌和玫烟色棒束孢对柑橘木虱成虫的LC50值分别为3.7×105和1.2×106 mL-1。同一孢子浓度对柑橘木虱的致死率室内效果优于半田间。2种真菌制剂对柑橘木虱成虫的致死时间与孢子浓度有关,LT50随着真菌孢子悬浮液浓度的增加而递减,球孢白僵菌孢子浓度为1×105~1×108mL-1时,柑橘木虱成虫的LT50值为5.2~4.4 d;玫烟色棒束孢孢子浓度为1×106~1×108 mL-1时,柑橘木虱成虫的LT50值为5.3~4.9 d。【结论】球孢白僵菌和玫烟色棒束孢菌株对柑橘木虱有良好的生物防治效果,柑橘木虱各虫态的死亡率与病原真菌的孢子浓度正相关。

    Abstract:

    【Objective】 To study pathogenicity and biological control effects of two entomopathogenic fungi Beauveria bassiana and Isaria fumosorosea to the nymphs and adults of Asian citrus psyllid (ACP) Diaphorina citri. 【Method】 The spore suspensions of B. bassiana and I. fumosorosea were diluted to 1×104, 1×105, 1×106, 1×107 and 1×108mL-1, and then were sprayed on ACP younger instar, older instar nymphs and adults indoor to study the lethal effects and under semi-field conditions to study the control effects against ACP adult population. 【Result】 B. bassiana and I. fumosorosea had higher pathogenicity to younger nymphs (2-3 instar) than to older nymphs(4-5 instar). After seven days infection, the LC50 of B. bassiana and I. fumosorosea to ACP older nymphs were 3.6×104 and 5.2×104 mL-1, to ACP younger nymphs were 3.5×104 and 4.2×104 mL-1, and to ACP adults were 1.4×105 and 1.6×105 mL-1 respectively. Under semi-field conditions, the LC50 of B. bassiana and I. fumosorosea to ACP adults increased to 3.7×105 and 1.2×106 mL-1. The lethal effects of two fungi at the same spore concentration against ACP in semifield were lower than those indoor. Meanwhile, the lethal time of both fungi to ACP adults was correlated with spore concentration. The LT50 decreased with the increase of spore concentration, ranged between 5.2-4.4 days when the spore concentration of B. bassiana varied from 1×105 to 1×108mL-1, and ranged between 5.3-4.9 days when the spore concentration of I. fumosorosea varied from 1×106 to 1×108mL-1. 【Conclusion】 Both B. bassiana and I. fumosorosea have good biocontrol effects against ACP. The fatality rates of ACP are positively correlated with the concentrations of fungal spores.

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代晓彦,李翌菡,沈祖乐,许炜明,吴建辉,任顺祥,邱宝利.球孢白僵菌与玫烟色棒束孢制剂对柑橘木虱的防治[J].华南农业大学学报,2017,38(1):63-68

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  • 收稿日期:2016-03-15
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  • 在线发布日期: 2017-01-11