国家自然科学基金（31572421，31270044）；中科院 “西部之光” 人才培养计划重点项目（2013）
目的 旨在研究母猪饲粮添加脯氨酸对哺乳环江香猪血浆生化参数和氨基酸含量的影响。方法 选取15头配种后第16天的环江香猪，随机分成3组，每组5个重复（栏），每个重复1头猪。分别饲喂添加w为0.77% L-丙氨酸（等氮对照组）、w为1.00% L-脯氨酸（脯氨酸组）、w为0.77% L-丙氨酸+w为0.0167%二氟甲基鸟氨酸（DFMO组）的试验饲粮。于产后第7和第14 天，每窝称体质量后随机选取1头哺乳仔猪，测定血浆生化参数和游离氨基酸含量。结果 7日龄时，脯氨酸组和DFMO组血氨浓度显著低于等氮对照组（P<0.05）。14日龄时，脯氨酸组仔猪体质量显著高于等氮对照组和DFMO组（P<0.05），血氨浓度和胆碱酯酶活性显著高于DFMO组（P<0.05），而肌酸激酶活性显著低于DFMO组（P<0.05），血浆葡萄糖和脯氨酸浓度显著高于等氮对照组（P<0.05）；脯氨酸组和DFMO组血浆精氨酸浓度显著高于等氮对照组（P<0.05）；等氮对照组和脯氨酸组血浆天冬酰胺浓度显著低于DFMO组（P<0.05）；DFMO组血浆鸟氨酸浓度显著高于等氮对照组（P<0.05）。结论 母猪饲粮添加脯氨酸可通过影响哺乳仔猪葡萄糖和氨基酸代谢而促进其生长发育。
Objective To investigate the effects of dietary proline supplementation on plasma biochemical parameters and amino acid in suckling Huanjiang mini-pigs.Method Fifteen female Huanjiang mini-pigs after 15 days of service were randomly divided into three groups, and reared in five pens (replicates) with one pig per pen. The pigs were fed with experimental diet supplementing 0.77% L-alanine (isonitrogenous control group), 1.00% L-proline (proline group), or 0.77% L-alanine+0.0167% 2-difluoromethylornithine (DFMO group). One piglet per litter was randomly selected on the 7th and 14th days respectively after weighing. The plasma biochemical parameters and free amino acid contents were analyzed.Result The concentrations of plasma ammonia in proline and DFMO groups at the age of 7 days were significantly lower than that in the isonitrogenous control group (P<0.05). At the age of 14 days, the proline group had significantly higher piglet body weight compared with the isonitrogenous control and DFMO groups (P<0.05), significantly higher plasma ammonia concentration and choline esterase (CHE) activity compared with the DFMO group (P<0.05), significantly lower creatine kinase (CK) activity compared with the DFMO group (P<0.05), and significantly higher concentrations of glucose and proline compared with the isonitrogenous control group (P<0.05).Arginine concentrations in proline and DFMO groups were significantly higher than that in the isonitrogenous control group (P<0.05). Asparagine concentrations in isonitrogenous control and proline groups were significantly lower than that in the DFMO group (P<0.05). Ornithine concentration in DFMO group was significantly higher than that in the isonitrogenous control group (P<0.05).Conclusion Dietary proline supplementation could improve the growth and development of suckling piglets by affecting the metabolism profiles glucose and amino acids.