四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究
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国家重点研发计划(2016YFD0501009);国家自然科学基金(31000548);成都大熊猫繁育研究基金(CPF2015-4,CPF2017-05);四川农业大学本科生科研兴趣培养项目(ky2016076)


Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas
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    摘要:

    【目的】了解四川省部分地区成年山羊贾第虫Giardia duodenalis感染情况以及感染虫种基因型。【方法】在四川省12个养殖场采集了342份成年山羊的新鲜粪便,采用多位点基因分型技术,通过巢式PCR扩增bgtpigdh基因位点,对阳性样品的PCR产物进行了测序分析。【结果】342份成年山羊粪样中贾第虫的总感染率为14.91%(51/342)。所有阳性样品感染的虫种基因型均为集聚体E。51份阳性样品在bgtpigdh这3个基因位点分别成功鉴定出4(E5、E8、E17、E18)、2(E2、E4)和2(E3、E4)种基因亚型,并且在bg位点发现了2种新的基因亚型(E17、E18)。【结论】四川省部分地区成年山羊存在较为严重的贾第虫感染,虫种类型主要为集聚体E,且具有一定的遗传多样性。

    Abstract:

    【Objective】 To investigate the infection states of Giardia duodenalis and the infected G. duodenalis genotype in adult goats in Sichuan partial areas.【Method】 The fecal samples of 342 adult goats were collected from 12 different farms of Sichuan, and subjected to nested PCR to amplify bg, tpi and gdh gene loci through multilocus genotyping technique. PCR products of positive samples were sequenced and further analyzed.【Result】 The infection rate of G. duodenalis in 342 adult goat fecal samples was 14.91% (51/342) and the infected G. duodenalis genotype in all positive samples was assemblage E. Fifty-one positive samples were respectively identified four (assemblage E5, E8, E17 and E18), two (assemblage E2 and E4), and two (assemblage E3 and E4) subtypes in bg, tpi and gdh gene loci. Two novel genotypes (assemblage E17 and E18) were detected at bg locus.【Conclusion】 There are serious G. duodenalis infection in adult goats in Sichuan partial areas. The infected G. duodenalis genotype is mainly assemblage E which has the genetic diversity at a certain level.

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涂蕊,但佳明,严光文,郑静,田一男,肖启程,魏斌,周紫峣,刘海峰,杨亭玉,彭广能,钟志军.四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究[J].华南农业大学学报,2018,39(5):59-64

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  • 收稿日期:2018-01-28
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  • 在线发布日期: 2018-07-18