目的 了解广东省球孢白僵菌Beauveria bassiana的群体遗传结构、基因流、菌株分化、遗传变异和寄主类型，以及与生态环境的相关性，为筛选优良生产菌株提供后备种质资源。方法 采用SSR分子标记对广东省鼎湖山国家级自然保护区（以下简称“DHS”）采集的81株球孢白僵菌进行遗传结构和多样性分析。结果 使用8对SSR引物扩增81个菌株产生多态位点数为58个，多态位点比率为100%，其中，引物Ba12多态位点数最多（10个）。通过Popgene 32软件包分析发现，DHS球孢白僵菌种群Nei’s基因多样性指数（h）为0.212 6，Shannon信息指数（Is）为0.348 7，可见其遗传多样性水平较高，群体异质性较强。将群体分别按照寄主和土壤、不同寄主目划分为不同亚群进一步分析，发现土壤亚群遗传多样性水平（h=0.192 5，Is=0.309 9）略高于寄主亚群（h=0.176 9，Is=0.278 3），种群间遗传分化程度较弱（Nm=1.662 9，Gst=0.130 7）；不同寄主目群体间的遗传分化较小且基因流明显。基于UPGMA聚类分析发现，各分支菌株的分布趋势与分离基质、不同寄主目均没有相关性。结论 DHS球孢白僵菌遗传谱系与分离基质和寄主来源均无明显相关性，不同生态环境和寄主类型的影响共同维持了DHS球孢白僵菌种群内遗传变异的多样性。
Objective To understand the population genetic structure, gene flow, strain differentiation of Beauveria bassiana in Guangdong Province, analyze the correlation between genetic variation and host type, ecological environment, and provide a large number of reserved germplasm resources for screening and production of superior strains.Method The genetic variation and diversity of 81 B. bassiana isolates from Dinghu Mountain National Nature Reserve of Guangdong Province (DHS) were assessed using SSR markers.Result Eight SSR primers were amplified to produce 58 polymorphic points, the percentage of polymorphic loci was 100%, and the number of polymorphic points of primer Ba12 was the largest (10). Nei's genetic diversity index (h) was 0.212 6 and Shannon's information index (Is) was 0.348 7 based on the analysis using the Popgene 32 software package, which indicated the genetic diversity and population heterogeneity levels of B. bassiana were high in DHS. Eighty-one strains of B. bassiana were divided into different subpopulations according to host/soil and insect order respectively. The genetic diversity of soil subpopulation (h=0.192 5, Is=0.309 9) was slightly higher than that of host subpopulation (h=0.176 9, Is=0.278 3), suggesting a generally low genetic differentiation among subpopulations (Nm=1.662 9, Gst=0.130 7).Genetic differentiation among subpopulations in different insect order was low and was associated with strong gene flow. The distribution of isolates had no obvious correlation with isolation medium or insect orders based on UPGMA clustering.Conclusion The genetic lineage of B. bassiana in DHS is not correlated with isolation medium or insect orders. The diversification of B. bassiana population in DHS is maintained by different ecological environment and host type.