目的 近年来受全球气候变暖等环境影响，我国亚热带区域森林虫害呈加剧态势，开展叶片生理性状与虫害程度关系研究具有重要意义。方法 采用典型样地调查，对刨花楠Machilus pauhoi和闽楠Phoebe bournei人工混交林受害植株健康叶片的非结构性物质含量、酶活性等主要生理性状与虫害等级关系进行研究。结果 虫害导致刨花楠和闽楠受害植株健康叶片的相对含水量显著上升，但对叶片干物质质量分数的影响并不明显。刨花楠和闽楠健康叶片可溶性糖质量分数在中度虫害时最低。刨花楠健康叶片可溶性蛋白质量分数随虫害等级的提高而降低，闽楠健康叶片可溶性蛋白质量分数在轻度虫害时最高。健康叶片总氨基酸质量分数最高出现在刨花楠轻度虫害和闽楠中度虫害时。二者可溶性蛋白和可溶性糖质量分数的比值均在中度虫害时达到最大值，但二者可溶性蛋白和可溶性糖质量分数比值的最小值出现在不同虫害等级。轻度和中度虫害的闽楠健康叶片可溶性蛋白与可溶性糖质量分数比值差异显著。2种楠树健康叶片的过氧化物酶（POD）活性总体呈先降后升的趋势；超氧化物歧化酶（SOD）活性则均以中度虫害时最高，重度虫害时最低，各等级间差异不显著。结论 2种楠树受相同虫害等级的影响并不总是具有一致性；2种楠树叶片酶活性对同等虫害的响应具有一致性。2种楠树的受害叶片和健康叶片具有相互补偿和自我调节作用，且2种楠树均具有自我愈伤能力和防御机制。研究结果可为进一步探讨未来环境变化下植物叶片对虫害的响应机制，开展人工林虫害防治技术研究等提供理论依据。
Objective Damages in subtropical regions of China caused by forest pests have been intensified due to environmental changes such as global warming. It is of great importance to study the relationship between leaf physiological traits and pest infestation grade.Method The relationships of main physiological traits, including non-structural substance contents and enzyme activities, and pest infestation grade on healthy leaves of Machilus pauhoi and Phoebe bournei artificial mixed forest were studied by typical plot survey.Result Pest damage caused significant increase in the relative water contents of healthy leaves of affected individuals, while did not have significant effect on leaf dry matter content. The soluble sugar contents of healthy leaves of M. pauhoi and P. bournei were the lowest in trees with moderate pest damage. The soluble protein content decreased in M. pauhoi healthy leaves with the increase of pest infestation grade. The soluble protein content of healthy leaves of P. bournei was the highest with slight pest damage. Total amino acid contents of healthy leaves of P. bournei and M. pauhoi were the highest with moderate pest damage and slight pest damage respectively. The ratios of soluble protein conents to soluble sugar contents reached the maximum in trees with moderate pest damage for both species, but the lowest values appeared in different pest levels for two species. The ratio of soluble protein content to soluble sugar content in leaves of P. bournei was significantly different between slight pest and moderate pest infestation grade. The peroxidase (POD) activities of healthy leaves of two species generally decreased first and then increased with the increase of pest infestation grade. The superoxide dismutase (SOD) activities were the highest under moderate pest infestation grade and the lowest under severe pest infestation grade, yet the differences among different pest infestation grade were not significant.Conclusion Two tree species have different response in non-structural substance contents to the same infestation grade of pest, while they have the same response in enzyme activities to the same infestation grade of pest. The damaged leaves and healthy leaves of two species have mutual compensation and self-regulation, and both species have self-healing ability and defense mechanism. The research results can provide a theoretical basis for further exploring the response mechanism of plant leaves to pests under future environmental changes and carrying out research on pest control techniques for artificial forest.