目的 研究生长素转运蛋白PIN基因家族在大豆Glycine max 根系适应低磷胁迫中的功能。方法 对大豆23个GmPIN家族成员进行进化树分析和表达模式分析，并进一步对GmPIN2b进行功能分析。结果 大豆23个GmPIN家族成员分散在7个不同的亚族，其中，GmPIN2a、GmPIN2b和GmPIN9a、GmPIN9d与拟南芥Arabidopsis thaliana的AtPIN2位于同一亚族。不同GmPIN家族成员在大豆中的组织表达定位及其受低磷调控的表达模式不同，其中，GmPIN2b在大豆根系中的表达水平在低磷胁迫6 d后显著上调，回补表达GmPIN2b能够部分恢复Atpin2突变体的表型。无论在低磷还是高磷条件下，回补表达GmPIN2b的转基因植株鲜质量和主根长均显著高于Atpin2突变体；而且回补表达GmPIN2b显著提高了Atpin2突变体在低磷条件下的一级侧根数，以及高磷条件下根系对重力的敏感性。结论 GmPIN2b在大豆根系形态建成响应低磷胁迫的过程中起重要的调控作用。
Objective To study the function of auxin transporter PIN gene family in the adaptation of soybean (Glycine max) roots to low phosphorus stress.Method A total of 23 GmPIN family members of soybean were conducted evolutionary tree and expression pattern analyses, so as to analyze the GmPIN2b function.Result The 23 GmPIN family members of soybean were scattered in seven different sub-families, among which GmPIN2a, GmPIN2b and GmPIN9a, GmPIN9d were in the same subfamily as Arabidopsis thaliana AtPIN2. Different GmPIN family members had different tissue expression locations in soybean and their expression patterns regulated by low phosphorus were different. Among them, the expression level of GmPIN2b in soybean roots was significantly up-regulated after low phosphorus stress for six days. The complement expression of GmPIN2b could partially restore the phenotype of Atpin2 mutants. The fresh weight and main root length of transgenic plants expressing GmPIN2b were significantly higher than those of Atpin2 mutant under both low or high phosphorus conditions. Moreover, the complement expression of GmPIN2b also significantly increased the number of primary lateral roots of Atpin2 mutant in low phosphorus treatment, and increased the sensitivity of the root system to gravity in high phosphorus treatment.Conclusion GmPIN2b plays an important regulatory role in the process of root morphogenesis in response to low phosphorus stress.