Ghd7DTH8协同调控水稻抽穗期和部分农艺性状
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国家自然科学基金(31871596,31921004)


Synergistic regulation of rice heading date and some agronomic traits by Ghd7 and DTH8
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    摘要:

    目的 抽穗期是水稻重要的农艺性状,决定品种适宜的栽培地区和季节。Ghd7DTH8是水稻的关键抽穗基因,全面揭示Ghd7DTH8在不同背景中的遗传互作规律、表达调控和对农艺性状的作用,对指导水稻品选育、提高水稻适应性和产量具有重要的应用价值。方法 本研究通过分子标记辅助选择从杂交稻‘宁优1179’后代中鉴定培育了1个hd1prr37无功能的背景下Ghd7DTH8的双基因分离群体,对其进行了抽穗期调查、下游基因(Ehd1Hd3aRFTI)表达检测和部分农艺性状分析。结果 在自然长日和自然短日下,Ghd7DTH8单基因均可抑制Ehd1Hd3aRFTI的表达,延迟抽穗,Ghd7DTH8单基因可以显著增加水稻株高、一级枝梗数、二级枝梗数、主穗粒数和千粒质量;Ghd7DTH8双基因组合强烈抑制Ehd1-Hd3a/RFT1通路的表达,延迟抽穗,并且能够显著增加水稻株高、一级枝梗数、二级枝梗数、主穗粒数和千粒质量等农艺性状。结论 长、短日下,Ghd7DTH8能协同抑制成花素基因表达,延长水稻抽穗期,正向调控多个产量相关性状,在水稻高产育种和遗传改良中具有重要的应用价值。

    Abstract:

    Objective Heading date is an important agronomic trait in rice, which determines suitable cultivated areas and seasons of rice varieties. Ghd7 and DTH8 are two key heading date genes in rice, comprehensively discovering their genetic interaction, expression regulation, and effects on agronomic traits are important for variety breeding, improvement of adaptability and yield of rice. Method In this study, we bred a Ghd7/DTH8 digenic segregating population in the non-functional hd1 and prr37 background from the progeny of the hybrid rice ‘Ningyou 1179’ by molecular marker-assisted selection. Heading date, expression of the downstream genes (Ehd1, Hd3a, RFT1), and some agronomic traits were investigated. Result Under natural long day and natural short day conditions, either Ghd7 or DTH8 independently repressed the expression of Ehd1, Hd3a and RFT1 genes, and delayed heading. Both Ghd7 and DTH8 could significantly promoted plant height, number of primary branches, number of secondary branches, spikelets of main panicle and 1000-grain weight. The Ghd7/DTH8 combination strongly suppressed the expression of the Ehd1-Hd3a/RFT1 pathway, and largely delayed heading. It also played a significant role to promote some agronomic traits, including plant height, number of primary branches, number of secondary branches, spikelets of main panicle and 1000-grain weight. Conclusion Ghd7/DTH8 synergistically represses the expression of the florigen genes, suppresses heading, and positively regulates some yield-related traits regardless of day-length, which is of great value for high yield breeding and genetic improvement of rice.

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肖冬冬,宗伍辈,孙康莉,李加俊,刘耀光,郭晶心.Ghd7DTH8协同调控水稻抽穗期和部分农艺性状[J].华南农业大学学报,2022,43(3):18-25

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  • 收稿日期:2021-09-30
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  • 在线发布日期: 2022-05-10