杨莉,刘宇航,郝佳,勾颖,潘根兴,杨利民.生物质炭对人参连作土壤微生物组成及功能的影响[J].华南农业大学学报,2022,43(1):28-36
生物质炭对人参连作土壤微生物组成及功能的影响
Effect of biochar on microbial composition and function in continuous cropping ginseng soil
投稿时间:2021-05-02  
DOI:10.7671/j.issn.1001-411X.202105001
中文关键词:  生物质炭  细菌分类  优势属  功能预测  连作障碍
英文关键词:Biochar  Bacterial classification  Dominant genera  Function prediction  Continuous cropping obstacle
基金项目:吉林省科技发展计划(20190304022YY,20200402104NC);国家重点研发计划(2019YFC1710700)
作者单位E-mail
杨莉 吉林农业大学 中药材学院/省部共建生态恢复与生态系统管理国家重点实验室培育基地吉林 长春 130118  
刘宇航 吉林农业大学 中药材学院/省部共建生态恢复与生态系统管理国家重点实验室培育基地吉林 长春 130118  
郝佳 吉林农业大学 中药材学院/省部共建生态恢复与生态系统管理国家重点实验室培育基地吉林 长春 130118  
勾颖 吉林农业大学 中药材学院/省部共建生态恢复与生态系统管理国家重点实验室培育基地吉林 长春 130118  
潘根兴 南京农业大学 农业资源与生态环境研究所江苏 南京 210095  
杨利民 吉林农业大学 中药材学院/省部共建生态恢复与生态系统管理国家重点实验室培育基地吉林 长春 130118 ylmh777@126.com 
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中文摘要:
      目的 探究栽培人参Panax ginseng C. A. Mey.后土壤细菌群落多样性与功能变化,以及增施生物质炭对土壤细菌群落多样性与功能的影响。方法 对人参连作土壤进行不同生物质炭改良的田间试验,结合高通量测序技术,分析土壤细菌群落多样性与功能变化。结果 植参土壤(前茬作物为三年生人参)中Spartobacteria_genera_incertae_sedis、Sphingomonas、Gemmatimonas、 Afipia 、Gp1、Gp2、Gp3、Gp6、Rummeliibacillus 9个属细菌数量显著降低,表明栽培人参或人参根系分泌物可能抑制上述细菌的生长。另外,植参土壤Gaiella属细菌数量显著增加,添加生物质炭后该属优势度进一步增加;说明栽培人参或人参根系分泌物以及施用生物质炭均可促进该细菌的生长。相较于新林土,植参土壤细菌数量、群落多样性、细菌分类组成及优势属占比均不同程度地下降,单优势种群比例上升。生物质炭处理对上述劣变趋势具有一定的正向调节作用,细菌分类组成及数量变化趋势趋向于新林土,并协助提升细菌染色质结构和动力学,转录,复制、重组和修复,信号转导机制与细胞防御功能。结论 增施生物质炭能够提升植参土壤细菌多样性,调节细菌群落结构以及细菌功能,使老参地土壤朝良好的方向发展。研究结果为连作人参土壤的修复及人参栽培提供了理论参考。
英文摘要:
      Objective To explore the changes of soil bacterial community diversity and function after planting ginseng (Panax ginseng C. A. Mey.), and the effects of biochar application on soil bacterial community diversity and function. Method The field experiments with different biomass charcoals were conducted to improve the soil of continuous cropping ginseng field, and the changes of bacterial community diversity and function were analyzed by high-throughput sequencing technology.Result The bacteria number of Spartobacteria_genera_incertae_sedis, Sphingomonas, Gemmatimonas, Afipia, Gp1, Gp2, Gp3, Gp6, and Rummeliibacillus in the soil after planting ginseng significantly decreased, indicating that cultivating ginseng or ginseng root secretions might inhibit the growth of these bacteria. In addition, the number of Gaiella bacteria in soil of planting ginseng field increased significantly, and further increased after applying biochar, indicating that all cultivating ginseng, ginseng root exudates and applying biochar could promote the growth of bacteria. Compared with the new forest soil, the number of soil bacteria, community diversity, bacterial taxonomic composition and the proportion of dominant genera in the continuous cropping ginseng field declined to varying degrees, and showed an increase in proportion of single dominant population. Biomass charcoal treatment had a certain positive regulation effect on the above-mentioned deterioration trend, and the change trend of bacterial classification and quantity tends to those of the new forest soil. The biomass charcoal assisted in improving bacteria chromatin structure and dynamics, transcription, replication recombination and repair, signal transduction mechanism and cell defense function. Conclusion Biochar application can improve soil bacterial diversty, regulate bacterial community structure and function, and make soil development of continuous cropping ginseng field in a good direction. The result provides therotical reference for soil restoration and ginseng cultivation in the continuous cropping ginseng field.
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