The rice blast resistance (R) could be evaluated entirely by investigating the resistance spectrum at seedling stage and neck blast resistance at adult stage. In this study, three space-induced rice mutant lines, which had been screened primarily, were performed with resistance spectra with 36 blast isolates from Guangdong Province and neck blast resistance in a natural blast nursery, Lütian, Conghua. The results indicated that the mutant lines conferred broad resistance spectra and high level of resistance to neck blast, while the on-ground control was highly susceptible. Genetic analysis showed that a single major dominant R gene conditioned H4’s and H11’s resistance to isolates GD0193 and GD866, and there were two independent dominant R genes in H4 and H11 corresponding to isoaltes GD08T4 and GD08T13, respectively. The inherited pattern of resistance in D69 to isolates GD0193 was complicated, while a single dominant major R gene controlled its resistance to isolate GD08T4 and GD866.