The great temperature difference present between the front and the rear of current fresh-keeping transportation makes negative impact on the quality of fruit and vegetable. In order to resolve this problem, double temperature sensors were applied in temperature control system designed in this paper, which utilized one temperature sensor to measure the temperature of the rear of preservation chamber as the prior value in controlled, and utilized the other temperature sensor to measure the temperature of the front as the auxiliary value in control. The system proved a good feasibility, stability by experiments, which could not only control the temperature under the set value in the preservation chamber, but also keep the temperature over the temperature limit of fruits and vegetables. This result is valuable for the improvement of the technology of the fresh-keeping transportation.