2025年1月23日 周四
头孢喹肟对金黄色葡萄球菌防突变浓度的研究
作者:
基金项目:

国家自然科学基金(31372480);广东省自然科学基金研究团队项目(S2012030006590)


A study on mutant prevention concentration of cefquinome against Staphylococcus aureus
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
    摘要:

    【目的】为兽医临床合理用药,进行头孢喹肟对金黄色葡萄球菌的最低抑菌浓度(MIC)和防突变浓度(MPC)研究.【方法】采用标准琼脂二倍稀释法,测定头孢喹肟对金黄色葡萄球菌的MIC 和 MIC99(抑制99%接种细菌生长的最低抑菌浓度);使用肉汤法富集1010 CFU/mL金黄色葡萄球菌ATCC 29213和37 株金黄色葡萄球菌临床分离菌,采用标准琼脂平板稀释法测定头孢喹肟对金黄色葡萄球菌的MPC,并计算选择指数(SI).【结果和结论】头孢喹肟对金黄色葡萄球菌ATCC 29213的MIC 和 MIC99 分别是0.5和0.4 μg/mL,MPC为1.6 μg/mL,SI (MPC/MIC99)为4.头孢喹肟对 37 株临床分离的金黄色葡萄球菌的MIC50、MIC90 和 MPC90 分别是0.50、1.00和5.12 μg/mL;SI (MPC90/MIC90)为5.12.头孢喹肟对金黄色葡萄球菌具有较强的抗菌活性,SI也较小,通过调整给药方案可以减少耐药突变株的产生.

    Abstract:

    【Objective】 The minimal inhibitory concentration (MIC) and the mutant prevention concentration (MPC) of cefquinome against Staphylococcus aureus were measured for the rational use in veterinary medicine.【Method】 The MIC and MIC for 99% of input cells (MIC99) of cefquinome against S. aureus were determined by agar two-fold dilution method. The S. aureus strains were enriched in broth, and the bacterial concentrations were adjusted to 1010 colony forming units (CFU) per milliliter. The MPC of cefquinome against S. aureus was determined by agar plates dilution method, and the selection index (SI) was calculated. 【Result and conclusion】 The MIC, MIC99, MPC and SI (MPC/MIC99) of cefquinome against S. aureus strain ATCC 29213 were 0.5, 0.4 , 1.6 μg/mL and 4, respectively. The MIC50, MIC90, MPC90 and SI (MPC90/MIC90) of cefquinome against S. aureus clinical isolates were 0.50, 1.00, 5.12 μg/mL and 5.12, respectively. The results show that cefquinome has a higher activity against S. aureus and a smaller SI. Resistant mutant strains can be reduced by adjusting the dosage regimen.

    参考文献
    相似文献
    引证文献
引用本文

王静,单奇,黎菊凤,利斌,张少威,曾振灵.头孢喹肟对金黄色葡萄球菌防突变浓度的研究[J].华南农业大学学报,2014,35(4):7-10

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2013-10-29
  • 在线发布日期: 2014-06-17